Tsuji T, Karasek M A
Acta Derm Venereol. 1986;66(6):474-8.
We have observed differential effects of the cytotoxicity of 5-fluorouracil (5-FU) on human skin melanocytes and malignant melanoma cells in vitro. In the absence of 5-FU the melanoma cells multiplied much more rapidly than the melanocytes. With a 7-day exposure of 5-FU (1.92 X 10(-5) to 3.84 X 10(-4) M) all the melanoma cells died by 5 W, while even two times longer exposure (a 14-day exposure) of 5-FU the melanocytes survived till 6 W and increased at the lower concentration. In the presence of 5-FU there was a different compensatory increase in [3H] thymidine incorporation between the two cell types. That is, compared with the large increase observed in the incorporation by the melanoma cells, a small increase in the incorporation by the melanocytes was observed. The reason for these differential effects of 5-FU may be the difference in the cell cycle and the post-injury cell renewal of the two cell types.
我们已经观察到5-氟尿嘧啶（5-FU）的细胞毒性在体外对人皮肤黑素细胞和恶性黑色素瘤细胞具有不同的影响。在没有5-FU的情况下，黑色素瘤细胞比黑素细胞增殖得快得多。用5-FU（1.92×10⁻⁵至3.84×10⁻⁴M）处理7天，所有黑色素瘤细胞在第5周时死亡，而即使将5-FU处理时间延长两倍（14天），黑素细胞在第6周时仍存活，并且在较低浓度下数量增加。在有5-FU存在的情况下，两种细胞类型之间[³H]胸苷掺入的代偿性增加情况不同。也就是说，与黑色素瘤细胞掺入量的大幅增加相比，黑素细胞掺入量的增加幅度较小。5-FU产生这些不同影响的原因可能是两种细胞类型在细胞周期和损伤后细胞更新方面存在差异。
