Center for Research and Development of Herbal Health Products, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
Phytother Res. 2014 Jul;28(7):1030-6. doi: 10.1002/ptr.5100. Epub 2013 Dec 11.
The florets of Carthamus tinctorius L. have traditionally been used for hair growth promotion. This study aimed to examine the potential of hydroxysafflor yellow A-rich C. tinctorius extract (CTE) on hair growth both in vitro and in vivo. The effect of CTE on cell proliferation and hair growth-associated gene expression in dermal papilla cells and keratinocytes (HaCaT) was determined. In addition, hair follicles from mouse neonates were isolated and cultured in media supplemented with CTE. Moreover, CTE was applied topically on the hair-shaved skin of female C57BL/6 mice, and the histological profile of the skin was investigated. C. tinctorius floret ethanolic extract promoted the proliferation of both dermal papilla cells and HaCaT and significantly stimulated hair growth-promoting genes, including vascular endothelial growth factor and keratinocyte growth factor. In contrast, CTE suppressed the expression of transforming growth factor-β1 that is the hair loss-related gene. Furthermore, CTE treatment resulted in a significant increase in the length of cultured hair follicles and stimulated the growth of hair with local effects in mice. The results provided the preclinical data to support the potential use of CTE as a hair growth-promoting agent.
红花的小花 traditionally 一直被用于促进头发生长。本研究旨在考察富含羟基红花黄色素 A 的红花提取物 (CTE) 在体外和体内促进头发生长的潜力。测定了 CTE 对真皮乳头细胞和角质形成细胞 (HaCaT) 中细胞增殖和与头发生长相关基因表达的影响。此外，从新生小鼠的毛囊中分离出来并在添加 CTE 的培养基中培养。此外，将 CTE 局部应用于雌性 C57BL/6 小鼠的剃毛皮肤上，研究皮肤的组织学特征。红花小花的乙醇提取物促进了真皮乳头细胞和 HaCaT 的增殖，并显著刺激了血管内皮生长因子和角质形成细胞生长因子等促进头发生长的基因的表达。相比之下，CTE 抑制了与脱发相关的基因转化生长因子-β1 的表达。此外，CTE 处理导致培养的毛囊长度显著增加，并在小鼠中具有局部作用刺激毛发生长。这些结果提供了支持 CTE 作为促进头发生长剂的潜在用途的临床前数据。
