Guo Xiao-Long, Lu Xi-Li, Dong De-Li, Sun Zhi-Jie
Center for Biomedical Materials and Engineering, Harbin Engineering University, 145 Nantong Street, Nangang District, Harbin, 150001, People's Republic of China.
J Biomed Mater Res A. 2014 Nov;102(11):3903-7. doi: 10.1002/jbm.a.35066. Epub 2013 Dec 21.
Poly(glycerol-sebacate) (PGS) is an elastomeric biodegradable polyester. Our previous series of studies have showed that PGS has good biocompatibility. In view of the potential use of PGS in bioengineering, we attempt to characterize the PGS polymer with different ratio of glycerol and sebacic acid, and the cell adhesion and growth on these polymers. PGSs with different proportion of glycerol and sebacic acid were synthesized by polycondensation reaction. The microstructure of the series PGSs were characterized by infrared spectroscopy and X-ray diffraction analysis (XRD). Results showed that, with the increase of the ratio of sebacic acid in PGS from 1:0.8, 1:1, to 1:1.2 (ratio of glycerol to sebacic acid), the main diffraction peak in XRD, the sol content and gel swelling increased but then decreased, suggesting that the degree of crosslinking and the inherent degree of order of the series PGS increased and then decreased. With the increase of sebacic acid proportion, water absorption increased and then decreased, and the water absorption ranged from 9.62% to 10.66%. The mass loss of the series of samples in degradation experiments ranged from 24.63% to 40.06% on the 32nd day of degradation. Cell culture data suggested that the polymer with the ratio of 1:0.8 for glycerol and sebacate was suitable for cell adhesion and growth. In conclusion, PGS can be used as the cell culture matrix by modifying the composition ratio of glycerol and sebacic acid to improve the properties of cell adhesion and growth.
聚（甘油-癸二酸酯）（PGS）是一种可生物降解的弹性体聚酯。我们之前的一系列研究表明，PGS具有良好的生物相容性。鉴于PGS在生物工程中的潜在应用，我们试图对不同甘油与癸二酸比例的PGS聚合物及其上的细胞黏附与生长情况进行表征。通过缩聚反应合成了不同甘油与癸二酸比例的PGS。采用红外光谱和X射线衍射分析（XRD）对该系列PGS的微观结构进行了表征。结果表明，随着PGS中癸二酸比例从1:0.8、1:1增加到1:1.2（甘油与癸二酸的比例），XRD中的主要衍射峰、溶胶含量和凝胶溶胀先增加后降低，表明该系列PGS的交联度和固有有序度先增加后降低。随着癸二酸比例的增加，吸水率先增加后降低，吸水率在9.62%至10.66%之间。在降解实验中，该系列样品在降解第32天时的质量损失在24.63%至40.06%之间。细胞培养数据表明，甘油与癸二酸比例为1:0.8的聚合物适合细胞黏附与生长。总之，通过改变甘油与癸二酸的组成比例来改善细胞黏附与生长性能，PGS可作为细胞培养基质。
