Ecole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, National Centre of Competence in Research (NCCR) in Chemical Biology, 1015 Lausanne (Switzerland).
Angew Chem Int Ed Engl. 2014 Jan 27;53(5):1302-5. doi: 10.1002/anie.201307754. Epub 2013 Dec 13.
Acetylcholine is a key neurotransmitter, and anticholinesterase agents are essential compounds used as medical drugs, pesticides, and chemical warfare agents. A semisynthetic fluorescence-based probe for the direct, real-time detection of acetylcholine and anticholinesterase compounds is introduced. The probe possesses good sensitivity, tunable detection range, and can be selectively targeted to cell surfaces, thereby making it an attractive tool for applications in analytical chemistry and quantitative biology.
乙酰胆碱是一种关键的神经递质，而乙酰胆碱酯酶抑制剂是作为药物、杀虫剂和化学战剂使用的重要化合物。本文介绍了一种基于半合成荧光的探针，用于直接、实时检测乙酰胆碱和乙酰胆碱酯酶化合物。该探针具有良好的灵敏度、可调的检测范围，并可选择性地靶向细胞表面，因此成为分析化学和定量生物学应用中极具吸引力的工具。
