Fuhrich Daniele G, Lessey Bruce A, Savaris Ricardo F
Department of Gynecology and Obstetrics, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of South Carolina School of Medicine-Greenville, Greenville, South Carolina, USA.
Anal Quant Cytopathol Histpathol. 2013 Aug;35(4):210-6.
To compare intraobserver and interobserver variation between traditional histological score (HSCORE) and digital HSCORE (D-HSCORE) performed by expert and naive researchers.
Immunohistochemical analysis of beta3 integrin subunit of 100 endometrial biopsies obtained from the midluteal phase of the menstrual cycle were reanalyzed using ImageJ software (D-HSCORE). Mean intensity of 3,3'-diaminobenzidine on endometrial glands was read by an expert (HSCORE) versus inexperienced observer using HSCORE and D-HSCORE.
The mean correlation [r(s)(95% CI)] between both methods was 0.86 (0.79-0.90) and highly significant (p < 0.0001) for the experienced individual. The naive researcher overestimated immunostaining, resulting (HSCORE) in negative samples. No discrepancies were seen with D-HSCORE. Interobserver variation for the inexperienced reader was 50% using HSCORE (cutoff 0.7) but 0% with D-HSCORE. Intraobserver variation using ImageJ was 0%.
The D-HSCORE performed by an inexperienced researcher has high correlation to traditional HSCORE performed by an expert.
比较专业研究人员和非专业研究人员使用传统组织学评分（HSCORE）和数字HSCORE（D-HSCORE）时的观察者内和观察者间差异。
使用ImageJ软件（D-HSCORE）对从月经周期黄体中期获取的100份子宫内膜活检组织的β3整合素亚基进行免疫组织化学分析。由一位专业人员（HSCORE）和一位经验不足的观察者分别使用HSCORE和D-HSCORE读取子宫内膜腺体上3,3'-二氨基联苯胺的平均强度。
对于有经验的个体，两种方法之间的平均相关性[r(s)(95% CI)]为0.86（0.79 - 0.90），且具有高度显著性（p < 0.0001）。非专业研究人员高估了免疫染色，导致（HSCORE）出现阴性样本。D-HSCORE未发现差异。经验不足的读者使用HSCORE（截断值0.7）时观察者间差异为50%，而使用D-HSCORE时为0%。使用ImageJ的观察者内差异为0%。
经验不足的研究人员使用的D-HSCORE与专业人员使用的传统HSCORE具有高度相关性。
