Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
Sci Total Environ. 2014 Feb 15;472:851-4. doi: 10.1016/j.scitotenv.2013.11.132. Epub 2013 Dec 15.
Nontuberculous mycobacteria (NTM) are ubiquitous organisms that have been isolated from a variety of environmental sources. Several NTM species are responsible for diseases in humans and/or animals known as mycobacterioses. The aim of this study was to determine the levels of NTM in the sediments and plants of five fish ponds in the Czech Republic using culture and quantitative real time PCR (qPCR). Additionally, we investigated if there was any link between environmental samples from the fish ponds and the fish occupying them. A total of 8 NTM (14.0%) were cultured from the aquatic environment. qPCR analysis showed that Mycobacterium avium hominissuis was most frequently present (54.4%), followed by Mycobacterium avium paratuberculosis (42.1%). The least frequently isolated NTM was Mycobacterium avium avium (5.3%). Thus, in this study we confirm the presence of mycobacteria in sediments and aquatic plants in fishponds, which are occupied by fish intended for human consumption. We successfully isolated NTM from the tissue of one fish and confirmed a possible transmission of mycobacteria from the aquatic environment to the fish. Consequently, the consumption of such fish represents a possible risk for consumers, particularly immunocompromised individuals.
非结核分枝杆菌（NTM）是一种普遍存在的生物体，已从各种环境源中分离出来。有几种 NTM 物种可导致人类和/或动物的疾病，称为分枝杆菌病。本研究的目的是使用培养和定量实时 PCR（qPCR）来确定捷克共和国五个鱼塘沉积物和植物中的 NTM 水平。此外，我们还研究了鱼塘的环境样本与其中的鱼类之间是否存在任何联系。共从水生环境中培养出 8 株 NTM（14.0％）。qPCR 分析显示，最常存在的是鸟分枝杆菌（Mycobacterium avium hominissuis）（54.4％），其次是副结核分枝杆菌（Mycobacterium avium paratuberculosis）（42.1％）。最少分离到的 NTM 是鸟分枝杆菌（Mycobacterium avium avium）（5.3％）。因此，在这项研究中，我们证实了鱼类养殖场沉积物和水生植物中存在分枝杆菌，这些鱼类是供人类食用的。我们成功地从一条鱼的组织中分离出了 NTM，并证实了分枝杆菌可能从水生环境传播到鱼类。因此，食用这些鱼类可能对消费者，特别是免疫功能低下的个体构成风险。
