Lin M C, Chao L S, Liou Y F
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1986 Feb;19(1):52-60.
A "sandwich" technique of enzyme immunoassay (EIA) kit for human alpha-fetoprotein has been developed by using commercially available reagents. The assay range is 0.5-60 ng/ml. Within-run coefficients of variation (C.V.) for 20 determination at three different concentrations were 4.4-10.3%, and between-run C.V. were 4.9-10.8%. The sensitivity and specificity of the kit has been compared with those of two commercially available kits (Abbott and Roche r1 = 0.991, r2 = 0.989.) This kit has some advantages over currently available kits. The assay is incubated in room temperature, no waterbath is required. The solid phase is dry store, it have long shelf life. The kit is inexpensive to operate, and it is suitable for any laboratory.
利用市售试剂开发了一种用于检测人甲胎蛋白的酶免疫分析（EIA）试剂盒的“夹心”技术。检测范围为0.5 - 60 ng/ml。在三个不同浓度下进行20次测定的批内变异系数（C.V.）为4.4 - 10.3%，批间变异系数为4.9 - 10.8%。已将该试剂盒的灵敏度和特异性与两种市售试剂盒（雅培和罗氏，r1 = 0.991，r2 = 0.989）进行了比较。该试剂盒相对于现有试剂盒具有一些优势。检测在室温下孵育，无需水浴。固相为干燥保存，保质期长。该试剂盒操作成本低，适用于任何实验室。
