采用酶联免疫吸附测定法测定血清甲胎蛋白。
Serum alphafetoprotein determination by enzyme-linked immunosorbent assay.
作者信息
Conradie J D, Gray R, Mbhele B E
出版信息
S Afr Med J. 1980 Jul 26;58(4):169-71.
PMID:6157201
Abstract
Enzyme-linked immunosorbent assay (ELISA) in microtitre trays has been adapted to the determination of alpha-fetoprotein (AFP); the method is described here. This assay has a lower limit of sensitivity of AFP 1 microgram/l and results compare well with those obtained with a commercial radio-immunoassay (RIA) kit (r = 0,994; y = 949x-3,00). ELISA has several advantages over RIA; it is considerably less expensive, the reagents are stable for longer periods and no elaborate equipment is required.
摘要
酶联免疫吸附测定（ELISA）已被应用于微量滴定板中α-甲胎蛋白（AFP）的测定；本文将对此方法进行描述。该测定法对AFP的检测下限为1微克/升，其结果与使用商业放射免疫测定（RIA）试剂盒所获得的结果具有良好的相关性（r = 0.994；y = 949x - 3.00）。与RIA相比，ELISA具有多个优点；它成本低得多，试剂稳定性更强，且无需精密设备。
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