硝酸甘油对模拟体外循环期间金黄色葡萄球菌生长及白细胞激活的影响

Effect of glyceryl trinitrate on staphylococcus aureus growth and leukocyte activation during simulated extracorporeal circulation.

作者信息

Melki Vilyam, Tano Eva, Douhan Hakansson Lena, Tran Phan-Kiet, Knutson Folke, Malinski Tadeusz, Borowiec Jan

机构信息

Department of Surgical Sciences, Uppsala University, Uppsala, Sweden.

Department of Medical Sciences, Uppsala University, Uppsala, Sweden.

出版信息

Thorac Cardiovasc Surg. 2014 Aug;62(5):402-8. doi: 10.1055/s-0033-1363296. Epub 2013 Dec 16.

DOI:10.1055/s-0033-1363296

PMID:24343379

Abstract

BACKGROUND

Previously, nitric oxide has been shown to possess antimicrobial effects. In this study, we aim to test the effect of glyceryl trinitrate (GTN) on Staphylococcus aureus growth during simulated extracorporeal circulation (SECC) and also to examine the effect of S. aureus, alone and in combination with GTN, on activation markers of the innate immune system during SECC.

METHODS

In an in vitro system of SECC, we measured GTN-induced changes in markers of leukocyte activation in whole blood caused by S. aureus infestation, as well as the effect of GTN on S. aureus growth.

RESULTS

GTN had no effect on S. aureus growth after 240 minutes SECC. Staphylococcus aureus reduced the expression of granulocyte Fcγ-receptor CD32 but stimulated the expression of monocyte CD32. Staphylococcus aureus stimulated expression of some leukocyte adhesion key proteins, activation marker CD66b, lipopolysaccharide-receptor CD14, and C3b-receptor CD35. Staphylococcus aureus and GTN addition induced significant increases in monocyte CD63 (lysosomal granule protein) levels.

CONCLUSION

GTN does not affect S. aureus growth during SECC and has no effect on SECC-induced leukocyte activation.

摘要

背景

此前已表明一氧化氮具有抗菌作用。在本研究中，我们旨在测试硝酸甘油（GTN）在模拟体外循环（SECC）期间对金黄色葡萄球菌生长的影响，并研究金黄色葡萄球菌单独及与GTN联合作用时对SECC期间固有免疫系统激活标志物的影响。

方法

在SECC的体外系统中，我们测量了GTN诱导的由金黄色葡萄球菌感染引起的全血中白细胞激活标志物的变化，以及GTN对金黄色葡萄球菌生长的影响。

结果

SECC 240分钟后，GTN对金黄色葡萄球菌生长无影响。金黄色葡萄球菌降低了粒细胞Fcγ受体CD32的表达，但刺激了单核细胞CD32的表达。金黄色葡萄球菌刺激了一些白细胞黏附关键蛋白、激活标志物CD66b、脂多糖受体CD14和C3b受体CD35的表达。添加金黄色葡萄球菌和GTN可使单核细胞CD63（溶酶体颗粒蛋白）水平显著升高。