Abbas Hisham A, Elsherbini Ahmed M, Shaldam Moutaz A
Department of Microbiology and Immunology-Faculty of Pharmacy-Zagazig University-Zagazig-Egypt.
Health Sciences College-Umm Al Qura University, AlQunfudah, Saudi Arabia.
Afr Health Sci. 2019 Mar;19(1):1376-1384. doi: 10.4314/ahs.v19i1.10.
is an important nosocomial bacterium that is responsible for a number of infections that may be fatal. The treatment of such infections is limited by emergence of antibiotic resistance. Targeting virulence of may provide an alternative option to antibiotic that may bypass the emergence of resistant strains due to lack of stress on viability.
Investigation of the ability of glyceryl trinitrate (GTN) to inhibit staphyloxanthin, biofilm and tolerance to oxidative stress.
The disk sensitivity method was used to detect the methicillin resistance of . The effect of sub-inhibitory concentration of GTN on biofilm formation, staphyloxanthin production and tolerance to oxidative stress was evaluated. Molecular docking study was used to investigate the ability of GTN to bind to dehydrosqualene synthase enzyme.
GTN showed a significant inhibition of biofilm, staphyloxanthin and tolerance to oxidative stress. In the molecular docking study, it was found that GTN could bind to dehydrosqualene synthase enzyme by hydrogen bonding, electrostatic interaction and pi-cation interaction.
The present study revealed the ability of GTN to serve as a potential anti-virulence candidate for attenuation of pathogenicity.
[细菌名称未给出]是一种重要的医院感染细菌，可导致多种可能致命的感染。此类感染的治疗因抗生素耐药性的出现而受到限制。针对[细菌名称未给出]的毒力进行靶向治疗可能为抗生素提供一种替代选择，由于对生存能力缺乏压力，可能避免耐药菌株的出现。
研究硝酸甘油（GTN）抑制[细菌名称未给出]的金黄色葡萄球菌黄素、生物膜形成及对氧化应激耐受性的能力。
采用纸片扩散法检测[细菌名称未给出]对甲氧西林的耐药性。评估亚抑菌浓度的GTN对生物膜形成、金黄色葡萄球菌黄素产生及对氧化应激耐受性的影响。利用分子对接研究来探究GTN与脱氢鲨烯合酶结合的能力。
GTN对生物膜、金黄色葡萄球菌黄素及对氧化应激的耐受性均有显著抑制作用。在分子对接研究中发现，GTN可通过氢键、静电相互作用和π-阳离子相互作用与脱氢鲨烯合酶结合。
本研究揭示了GTN作为一种潜在的抗毒力候选药物以减弱[细菌名称未给出]致病性的能力。
