Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
Vet Microbiol. 2014 Jan 31;168(2-4):428-31. doi: 10.1016/j.vetmic.2013.11.015. Epub 2013 Nov 22.
In the present study 13 Arcanobacterium pluranimalium strains isolated from various animal origin could successfully be identified phenotypically by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and the pluranimaliumlysin encoding gene pla. The detection of mass spectra by MALDI-TOF MS and the novel genotypic approach using gene pla might help to identify A. pluranimalium in future and might elucidate the role this species plays in infections of animals.
在本研究中,通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)对从各种动物来源分离的 13 株 Arcanobacterium pluranimalium 菌株进行了表型鉴定,通过 16S rDNA 和 pluranimaliumlysin 编码基因 pla 的测序进行了基因型鉴定。MALDI-TOF MS 检测质谱和使用 pla 基因的新型基因分型方法可能有助于未来鉴定 A. pluranimalium,并阐明该物种在动物感染中的作用。