Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Frankfurter Str. 107, 35392 Giessen, Germany.
Vet Microbiol. 2012 May 25;157(1-2):243-5. doi: 10.1016/j.vetmic.2011.12.022. Epub 2011 Dec 26.
In the present study matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for species identification of 98 bacteria previously classified phenotypically and genotypically to genera Arcanobacterium and Trueperella. Species identification was carried out by comparing the main spectra of each strain with the main spectra of reference strains of both genera and 3740 database entries included in the MALDI Biotyper 2.0 software package (Bruker Daltonik GmbH, Bremen, Germany). MALDI-TOF MS correctly identified (log (score) values ≥ 2.0) all investigated strains of the species A. (T.) bialowiezense (n=3), A. (T.) bonasi (n=7), A. haemolyticum (n=10), A. pluranimalium (n=1) and A. (T.) pyogenes (n=77). According to the present results MALDI-TOF MS had a comparable discriminating power than previously conducted tests on DNA level. Further studies with strains isolated from human infections would show the robustness of MALDI-TOF MS for identification of bacteria of these genera.
在本研究中，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）用于鉴定先前通过表型和基因型分类为 Arcanobacterium 和 Trueperella 属的 98 种细菌的种属。通过将每个菌株的主要图谱与两个属的参考菌株的主要图谱以及 MALDI Biotyper 2.0 软件包（德国不来梅布鲁克达尔通尼克有限公司）中包含的 3740 个数据库条目进行比较，进行了种属鉴定。MALDI-TOF MS 正确鉴定了（log（得分）值≥2.0）所有研究的 A.（T.）bialowiezense（n=3）、A.（T.）bonasi（n=7）、A.hemolyticum（n=10）、A. pluranimalium（n=1）和 A.（T.）pyogenes（n=77）菌株。根据目前的结果，MALDI-TOF MS 具有与先前在 DNA 水平上进行的测试相当的区分能力。进一步研究从人类感染中分离的菌株将表明 MALDI-TOF MS 对这些属细菌鉴定的稳健性。
