Risse Karin, Schlez Karen, Eisenberg Tobias, Geiger Christina, Balbutskaya Anna, Sammra Osama, Lämmler Christoph, Abdulmawjood Amir
Landesbetrieb Hessisches Landeslabor, Schubertstraße 60, 35392 Gießen, Germany.
Frankfurt Zoo, Bernhard-Grzimek-Allee 1, 60316 Frankfurt, Germany.
J Vet Med. 2014;2014:408724. doi: 10.1155/2014/408724. Epub 2014 Apr 30.
The present study was designed to characterize phenotypically and genotypically an Arcanobacterium pluranimalium strain (A. pluranimalium 4868) following necropsy from a juvenile giraffe. The species identity could be confirmed by phenotypical investigations and by MALDI-TOF MS analysis, by sequencing the 16S rDNA, pluranimaliumlysin encoding gene pla, and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap with sequence similarities to A. pluranimalium reference strain DSM 13483(T) of 99.2%, 89.9%, and 99.1%, respectively. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. pluranimalium strain isolated from a giraffe.
本研究旨在对一头幼年长颈鹿尸检后获得的多动物隐秘杆菌菌株(多动物隐秘杆菌4868)进行表型和基因型特征分析。通过表型研究、基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)分析、对16S rDNA、多动物溶素编码基因pla和甘油醛-3-磷酸脱氢酶编码基因gap进行测序,证实该菌株与多动物隐秘杆菌参考菌株DSM 13483(T)的序列相似性分别为99.2%、89.9%和99.1%,从而确定了该物种的身份。据我们所知,本研究是首次对从长颈鹿分离出的多动物隐秘杆菌菌株进行表型和基因型特征分析。