Yanagihara Y, Kajiwara K, Kiniwa M, Yui Y, Shida T, Delespesse G
J Allergy Clin Immunol. 1987 Mar;79(3):448-56. doi: 10.1016/0091-6749(87)90362-9.
Culture supernatants of unstimulated T cells (TCS) derived from normal donors or from atopic patients with bronchial asthma were tested for their ability to regulate the spontaneous IgE synthesis by B cells of normal and atopic subjects. The same TCS were also tested for their influence on the histamine release from leukocytes of house dust mites-sensitive patients. Addition of TCS to B cell cultures from allergic donors induced a dose-dependent increase of the spontaneous IgE production without affecting the synthesis of IgG, IgM, and IgA. The potentiating activity of TCS was observed only in B cell cultures spontaneously producing IgE; TCS were still active on irradiated B cells. The maximal IgE-enhancing activity was observed when TCS were added at the onset of B cell cultures. The supernatants of T cells lysed at day 0 did not contain IgE-potentiating factors. The antigen-induced but not the spontaneous histamine release from leukocytes of house dust mite-sensitive patients was enhanced by pretreatment with TCS from allergic donors. The enhancing activities of TCS on IgE synthesis and on histamine release could be removed by absorption with IgE-Sepharose and subsequently recovered by elution with glycine buffer. The results indicate that T cells of patients with asthma spontaneously release IgE-binding factors capable of increasing both the spontaneous IgE synthesis by B cells and the antigen-induced histamine release.
对来自正常供体或患有支气管哮喘的特应性患者的未刺激T细胞的培养上清液（TCS）进行检测，以评估其调节正常和特应性受试者B细胞自发合成IgE的能力。还检测了相同的TCS对屋尘螨敏感患者白细胞组胺释放的影响。向来自过敏供体的B细胞培养物中添加TCS会导致自发IgE产生呈剂量依赖性增加，而不影响IgG、IgM和IgA的合成。仅在自发产生IgE的B细胞培养物中观察到TCS的增强活性；TCS对经辐射的B细胞仍有活性。当在B细胞培养开始时添加TCS时，观察到最大的IgE增强活性。第0天裂解的T细胞上清液不含IgE增强因子。来自过敏供体的TCS预处理可增强屋尘螨敏感患者白细胞由抗原诱导而非自发的组胺释放。TCS对IgE合成和组胺释放的增强活性可通过用IgE-琼脂糖吸附去除，随后用甘氨酸缓冲液洗脱回收。结果表明，哮喘患者的T细胞自发释放能够增加B细胞自发IgE合成以及抗原诱导组胺释放的IgE结合因子。
