采用 LC/MS 和 HPLC 对苦参中的黄酮类化合物进行定性和定量分析。

Qualitative and quantitative analysis of flavonoids in Sophora tonkinensis by LC/MS and HPLC.

机构信息

Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China.

出版信息

Chin J Nat Med. 2013 Nov;11(6):690-8. doi: 10.1016/S1875-5364(13)60081-3.

DOI:10.1016/S1875-5364(13)60081-3

PMID:24345512

Abstract

AIM

To develop analytical methods for the identification and determination of the flavonoids in Sophora tonkinensis for comprehensive quality evaluation.

METHODS

An HPLC-DAD-ESI-MS method was used for the separation and characterization of the flavonoids in S. tonkinensis, and a liquid chromatographic method was employed to simultaneously determine five major active flavonoids in this crude drug.

RESULTS

Seventeen flavonoids were identified, among which, seven were unambiguously identified as trifolirhizin, quercetin, formononetin, macckiain, kurarinone, sophoranone, and sophoranochromene by comparing their retention times, and UV and MS spectra with those of the authentic compounds, and the other ten flavonoids were tentatively identified by comparing their UV and MS/MS spectra with those of literature data. Furthermore, five major active flavonoids, including trifolirhizin, quercetin, maackiain, sophoranone, and sophoranochromene were determined in S. tonkinensis. All calibration curves expressed good linearity (r > 0.999 8) within the test ranges, and the recovery from this method was 96.40%-104.43%. The developed HPLC method was successfully applied to quantitatively determine the five flavonoids in seventeen samples of S. tonkinensis.

CONCLUSION

The developed method rapidly characterized the bioactive flavonoids of S. tonkinensis, and could be readily utilized to enhance the quality assurance approaches for this traditional Chinese medicine.

摘要

目的

建立分析方法，用于鉴定和测定苦参中的黄酮类化合物，以进行综合质量评价。

方法

采用高效液相色谱-二极管阵列检测-电喷雾离子阱质谱联用（HPLC-DAD-ESI-MS）法分离和鉴定苦参中的黄酮类化合物，并采用液相色谱法同时测定该药材中 5 种主要活性黄酮类化合物。

结果

鉴定出 17 种黄酮类化合物，其中 7 种通过与对照品比较保留时间、紫外和质谱图谱，明确鉴定为三叶豆紫檀苷、槲皮素、芒柄花素、马钱苷、苦参酮、槐定碱和槐烷型色烯；其余 10 种黄酮类化合物通过与文献数据比较紫外和质谱二级图谱，初步鉴定。此外，在苦参中还测定了 5 种主要活性黄酮类化合物，包括三叶豆紫檀苷、槲皮素、马钱苷、苦参酮和槐烷型色烯。所有校准曲线在测试范围内均表现出良好的线性（r>0.999 8），该方法的回收率为 96.40%-104.43%。所建立的 HPLC 方法成功地用于定量测定 17 份苦参样品中的 5 种黄酮类化合物。