叶提取物对大鼠切口感染的影响：抗氧化、抗菌和基因表达分析。

Effect of Leaf Extract on Excision Wound Infections in Rats: Antioxidant, Antimicrobial, and Gene Expression Analysis.

机构信息

Department of Clinical Laboratory Science, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi Arabia.

出版信息

Molecules. 2022 Jul 13;27(14):4481. doi: 10.3390/molecules27144481.

The present study investigated the wound healing activity of leaf extract on an infected excision wound model in rats. Infection was induced using methicillin-resistant (MRSA) or An investigation was also done to study the effect of extract on the vascular endothelial growth factor (VEGF) and transforming growth factor-beta 1 (TGF-β1) gene expression in vitro using human keratinocytes (HaCaT). The methanol extract of leaves was analyzed for the presence of phytochemicals by LCMS. The antimicrobial activity of the extract was also determined. Wound contraction, days for epithelization, antioxidant enzyme activities, epidermal height, angiogenesis, and collagen deposition were studied. showed an antimicrobial effect and significantly improved wound contraction, reduced epithelization period, increased antioxidant enzymes activity, and reduced capillary density. Effect of the extract was less in wounds infected with when compared to MRSA. The VEGF and TGF-β1 gene expression was increased by .

本研究调查了叶提取物对大鼠感染性切口创面模型的愈合活性。采用耐甲氧西林金黄色葡萄球菌（MRSA）或感染诱导。还进行了一项研究，以调查提取物对体外人角质形成细胞（HaCaT）中血管内皮生长因子（VEGF）和转化生长因子-β1（TGF-β1）基因表达的影响。通过 LCMS 分析叶甲醇提取物中是否存在植物化学物质。还测定了提取物的抗菌活性。研究了伤口收缩、上皮化天数、抗氧化酶活性、表皮高度、血管生成和胶原沉积。表现出抗菌作用，显著促进伤口收缩，减少上皮化时间，增加抗氧化酶活性，减少毛细血管密度。与 MRSA 相比，提取物对感染的伤口的作用较小。增加了 VEGF 和 TGF-β1 基因的表达。