School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA; Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA.
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
Biomaterials. 2014 Mar;35(8):2477-87. doi: 10.1016/j.biomaterials.2013.11.044. Epub 2013 Dec 15.
The performance of biomaterials-based therapies can be hindered by complications associated with surgical implant, motivating the development of materials systems that allow minimally invasive introduction into the host. In this study, we created cell-adhesive and degradable gelatin scaffolds that could be injected through a conventional needle while maintaining a predefined geometry and architecture. These scaffolds supported attachment, proliferation, and survival of cells in vitro and could be degraded by recombinant matrix metalloproteinase-2 and -9. Prefabricated gelatin cryogels rapidly resumed their original shape when injected subcutaneously into mice and elicited only a minor host response following injection. Controlled release of granulocyte-macrophage colony-stimulating factor from gelatin cryogels resulted in complete infiltration of the scaffold by immune cells and promoted matrix metalloproteinase production leading to cell-mediated degradation of the cryogel matrix. These findings suggest that gelatin cryogels could serve as a cell-responsive platform for biomaterial-based therapy.
生物材料疗法的性能可能会受到与手术植入相关的并发症的阻碍，这促使人们开发出允许微创引入宿主的材料系统。在这项研究中，我们创建了具有细胞黏附性和可降解性的明胶支架，这些支架可以通过常规的针管进行注射，同时保持预设的几何形状和结构。这些支架支持细胞在体外的附着、增殖和存活，并且可以被重组基质金属蛋白酶-2 和 -9 降解。预制的明胶冷冻凝胶在皮下注射到小鼠体内后迅速恢复到原来的形状，并且在注射后仅引起轻微的宿主反应。从明胶冷冻凝胶中控制释放粒细胞-巨噬细胞集落刺激因子导致支架完全被免疫细胞浸润，并促进基质金属蛋白酶的产生，导致细胞介导的冷冻凝胶基质降解。这些发现表明，明胶冷冻凝胶可以作为一种细胞响应的生物材料治疗平台。
