Torseth J W, Nickoloff B J, Basham T Y, Merigan T C
J Infect Dis. 1987 Apr;155(4):641-8. doi: 10.1093/infdis/155.4.641.
beta Interferon (IFN) was demonstrated by specific, sequential antibody-neutralization assays of vesicle fluids from patients with recurrent skin lesions due to herpes simplex virus. To determine the origin of this antiviral activity, we cultured keratinocytes from normal facial skin and infected them with three strains of herpes simplex virus. Keratinocyte cultures then developed characteristic cytopathic changes, and antiviral activity was found in culture supernatant media. All such activity from these supernatants was neutralized with specific antiserum to IFN-beta but not with antiserum to IFN-alpha. No IFN-gamma was detectable by radioimmunoassay. Immunoperoxidase staining with antiserum to IFN-beta in five biopsy specimens from culture-proven, recurrent herpes simplex lesions showed positive staining of epidermal keratinocytes but not of dermal or infiltrating cells. Thus, the primary sources of IFN-beta in recurrent herpes lesion vesicles are the virus-infected keratinocytes.
通过对单纯疱疹病毒引起的复发性皮肤病变患者的囊泡液进行特异性、序贯抗体中和试验，证实了β干扰素（IFN）的存在。为了确定这种抗病毒活性的来源，我们培养了来自正常面部皮肤的角质形成细胞，并用三株单纯疱疹病毒感染它们。角质形成细胞培养物随后出现特征性细胞病变变化，并且在培养上清液中发现了抗病毒活性。这些上清液中的所有此类活性均被针对IFN-β的特异性抗血清中和，但未被针对IFN-α的抗血清中和。通过放射免疫测定法未检测到IFN-γ。用针对IFN-β的抗血清对来自经培养证实的复发性单纯疱疹病变的五个活检标本进行免疫过氧化物酶染色，结果显示表皮角质形成细胞呈阳性染色，而真皮或浸润细胞未呈阳性染色。因此，复发性疱疹病变囊泡中IFN-β的主要来源是病毒感染的角质形成细胞。
