Schnipper L E, Levin M, Crumpacker C S, Gilchrest B A
J Invest Dermatol. 1984 Jan;82(1):94-6. doi: 10.1111/1523-1747.ep12259193.
Keratinocyte cultures derived from surgical skin specimens of healthy newborns and adults were infected with herpes simplex virus (HSV) type 1 or 2. Typical HSV cytopathic effects involved all cell layers in stratified colonies, and paralleled the production of infectious virus. Virus growth curves and production of virus were comparable in newborn and adult keratinocytes. Interferon (IF) production by keratinocytes paralleled the yield of virus over at least 72 h, and was greater in cultures of adult cells than cultures from newborns. UV irradiation of HSV resulted in progressive virus inactivation and a parallel reduction in induced IF. This suggests that IF production was related to virus replication, and that irradiated (noninfectious) HSV DNA did not contribute significantly to the generation of IF in this system. These results establish that human epidermal keratinocytes can serve as a model system for quantitative assessment of herpes simplex virus infection.
取自健康新生儿和成人手术皮肤标本的角质形成细胞培养物，用1型或2型单纯疱疹病毒（HSV）进行感染。典型的HSV细胞病变效应累及分层菌落中的所有细胞层，且与传染性病毒的产生平行。新生儿和成人角质形成细胞中的病毒生长曲线及病毒产生情况相当。角质形成细胞产生的干扰素（IF）在至少72小时内与病毒产量平行，且在成人细胞培养物中比新生儿细胞培养物中的产量更高。对HSV进行紫外线照射导致病毒逐渐失活，并使诱导产生的IF平行减少。这表明IF的产生与病毒复制有关，且在该系统中，经照射（无感染性）的HSV DNA对IF的产生贡献不大。这些结果表明，人表皮角质形成细胞可作为定量评估单纯疱疹病毒感染的模型系统。
