Chapman S K, Hoover M S
Am J Physiol. 1978 Mar;234(3):F235-7. doi: 10.1152/ajprenal.1978.234.3.F235.
The effect of acetazolamide on ammonia-producing enzyme systems was determined in vitro at concentrations comparable to those which have been shown to abolish ammonium excretion in vivo. No change in the activity of glutaminase or gamma-glutamyl transpeptidase could be observed at concentrations up to 0.2 mM acetazolamide, and concentrations up to 1 mM were without effect on D-glutamyltransferase activity. Therefore, the effect of acetazolamide to abolish ammonium excretion cannot be explained by an action of the drug to inhibit ammoniagenesis.
在体外测定了乙酰唑胺对产氨酶系统的影响，其浓度与体内已证实可消除铵排泄的浓度相当。在乙酰唑胺浓度高达0.2 mM时，未观察到谷氨酰胺酶或γ-谷氨酰转肽酶活性的变化，浓度高达1 mM时对D-谷氨酰转移酶活性也无影响。因此，乙酰唑胺消除铵排泄的作用不能用该药物抑制氨生成的作用来解释。
