Aderounmu A O, Omonisi A E, Akingbasote J A, Makanjuola M, Bejide R A, Orafidiya L O, Adelusola K A
Department of Surgery, Ladoke Akintola University of Technology Teaching Hospital, Osogbo, Nigeria.
Afr J Tradit Complement Altern Med. 2013 Apr 12;10(3):574-9. eCollection 2013.
Calotropis Procera (CP) has been used in the management of toothache, fresh skin burns, gum bleeding as well as others to make it qualify as a medicinal plant. This study was designed to assess its wound-healing property in rabbits and its potentials for anti keloidal activity.Fresh latex of Calotropis were obtained and evaluated phytochemically. Fifteen male rabbits were used and four excisional wounds were created on each rabbit. The rabbits were divided into five groups of three each. Group 1 was the negative control and received no treatment. The wounds of group 2 animals were treated with 2mL of Calotropis latex; group 3 with 2mL honey; and group 4 with a mixture of 1ml honey and 1 mL of the latex. The animals in group 5 were given 2mg triamcinolone intramuscularly. All the groups had their wounds treated daily for 21 days. The wounds' diameters were measured on the day of wound creation, thereafter on days 7, 14 and 21 post wound creation. Biopsies of the wounds were taken on days 3 and 21 and viewed histologically. Phytochemical study of the latex revealed the presence of glycosides, tannins and alkaloids. The wounds were found to be significantly (p<0.05) reduced in groups treated with 50% latex in honey and triamcinolone, respectively, on day 7 post wound creation while there was a significant (p<0.05) reduction in wound surface area in all treated groups on days 14 and 21 post wound creation. Histological findings in untreated group showed thick bundle of collagen fibres some of which had broad based configurations, reminiscent of keloid. The group treated with 2mL of Calotropis latex revealed the presence of florid granulation tissues on day 3 while there was a marked reduction in quantity and size of collagen fibres on day 21 post wound creation which was comparable with what was seen for the triamcinolone-treated group.The general effect of Calotropis latex on wound-healing was noted. Likewise it's similarity to that of triamcinolone, an anti-keloidal agent; this makes it a probable candidate for future anti-keloidal study using a suitable model.
牛角瓜已被用于治疗牙痛、新鲜皮肤烧伤、牙龈出血等，使其有资格成为一种药用植物。本研究旨在评估其对家兔的伤口愈合特性及其抗瘢痕疙瘩活性的潜力。获取了牛角瓜的新鲜乳汁并进行了植物化学评估。使用了15只雄性家兔，每只家兔制造4个切除性伤口。将家兔分为5组，每组3只。第1组为阴性对照，不接受治疗。第2组动物的伤口用2mL牛角瓜乳汁处理；第3组用2mL蜂蜜处理；第4组用1mL蜂蜜和1mL乳汁的混合物处理。第5组动物肌肉注射2mg曲安奈德。所有组的伤口每天处理，持续21天。在伤口创建当天测量伤口直径，此后在伤口创建后的第7天、14天和21天测量。在第3天和第21天对伤口进行活检并进行组织学观察。乳汁的植物化学研究表明存在糖苷、单宁和生物碱。在伤口创建后第7天，分别用50%乳汁加蜂蜜处理组和曲安奈德处理组的伤口明显（p<0.05）缩小，而在伤口创建后第14天和21天，所有处理组的伤口表面积均有明显（p<0.05）减小。未处理组的组织学结果显示有厚的胶原纤维束，其中一些具有宽基构型，类似瘢痕疙瘩。用2mL牛角瓜乳汁处理的组在第3天显示有丰富的肉芽组织，而在伤口创建后第21天胶原纤维的数量和大小明显减少，这与曲安奈德处理组所见相当。注意到了牛角瓜乳汁对伤口愈合的总体作用。同样，它与抗瘢痕疙瘩药物曲安奈德相似；这使其有可能成为未来使用合适模型进行抗瘢痕疙瘩研究的候选药物。
