Department of Orthopedics, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.
Department of Orthopedics, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154000, China.
Evid Based Complement Alternat Med. 2013;2013:164817. doi: 10.1155/2013/164817. Epub 2013 Nov 24.
In the present study, we investigated the anti-inflammatory activity of water-soluble polysaccharide of Agaricus blazei Murill (WSP-AbM) on ovariectomized osteopenic rats. The rats were administered orally WSP-AbM (200 mg/kg BW) for 8 weeks. Subsequent serum maleic dialdehyde (MDA) level, total antioxidant status (TAOS), nuclear factor kappa B (NF- κ B) level, polymorphonuclear (PMN) cells level, interleukin-1 β (IL-1 β ) level, inducible nitric oxide synthase (iNOS) level, tumor necrosis factor- α (TNF- α ) level, adhesion molecule (ICAM-1), and cyclooxygenase-2 (COX-2) were determined by enzyme linked immunosorbent assay (ELISA) and immunohistochemistry, respectively. WSP-AbM administration markedly (P < 0.05) decreased serum IL-1 β and TNF- α levels and the expressions of ICAM-1, COX-2, and iNOS NF- κ B compared with OVX rats. WSP-AbM administration alsomarkedly (P < 0.05) decreased PMN infiltration. In conclusion, we observed that WSP-AbM supplementation had anti-inflammatory effects in a model of osteoporosis disease.
在本研究中，我们研究了姬松茸水溶性多糖（WSP-AbM）对去卵巢骨质疏松大鼠的抗炎活性。大鼠口服 WSP-AbM（200mg/kgBW）8 周。随后通过酶联免疫吸附试验（ELISA）和免疫组织化学法测定血清丙二醛（MDA）水平、总抗氧化状态（TAOS）、核因子-κB（NF-κB）水平、多形核（PMN）细胞水平、白细胞介素-1β（IL-1β）水平、诱导型一氧化氮合酶（iNOS）水平、肿瘤坏死因子-α（TNF-α）水平、细胞间黏附分子-1（ICAM-1）和环氧化酶-2（COX-2）。与 OVX 大鼠相比，WSP-AbM 给药显著降低了血清 IL-1β 和 TNF-α 水平以及 ICAM-1、COX-2 和 iNOS NF-κB 的表达。WSP-AbM 给药还显著减少了 PMN 浸润。综上所述，我们观察到 WSP-AbM 补充对骨质疏松症模型具有抗炎作用。
