Yu Lei, Yang Shaolong, Sun Lei, Jiang Yan-Fang, Zhu Li-Ying
Department of Infectious Disease, The Fourth Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang, People's Republic of China.
Biol Trace Elem Res. 2014 Jul;160(1):79-84. doi: 10.1007/s12011-014-0022-8. Epub 2014 Jun 8.
In the present study, we investigated the effects of Se-enriched Agaricus blazei Murill (Se-AbM) on liver injury in mice induced by acute alcohol administration. Mice received ethanol (5 g/kg body weight (BW)) by gavage every 12 h for a total of 3 doses. Se-AbM was administrated before ethanol administration. Subsequent serum alanine aminotransferase (ALT) level, aspartate aminotransaminase (AST) level, maleic dialdehyde (MDA) level, hepatic total antioxidant status (TAOS), nuclear factor kappa B (NF-κB) level, polymorphonuclear cells (PMN) level, interleukin-1β (IL-1β) level, inducible nitric oxide synthase (iNOS) level, tumor necrosis factor-α (TNF-α) level, intercellular adhesion molecule 1 (ICAM-1), and cyclooxygenase-2 (COX-2) were determined by ELISA and immunohistochemistry, respectively. Se-AbM administration markedly (p < 005) decreased serum ALT, AST, and MDA levels, hepatic IL-1β and TNF-α levels, as well as PMN infiltration and the expression of ICAM-1, COX-2, iNOS, and NF-κB compared with alcohol administration. In conclusion, we observed that Se-AbM supplementation could restrain the hepatic damage caused by acute alcohol exposure.
在本研究中，我们调查了富硒姬松茸（Se-AbM）对急性酒精给药诱导的小鼠肝损伤的影响。小鼠每12小时经口灌胃给予乙醇（5 g/kg体重（BW）），共3剂。在给予乙醇之前给予Se-AbM。随后分别通过酶联免疫吸附测定（ELISA）和免疫组织化学测定血清丙氨酸氨基转移酶（ALT）水平、天冬氨酸氨基转移酶（AST）水平、丙二醛（MDA）水平、肝脏总抗氧化状态（TAOS）、核因子κB（NF-κB）水平、多形核细胞（PMN）水平、白细胞介素-1β（IL-1β）水平、诱导型一氧化氮合酶（iNOS）水平、肿瘤坏死因子-α（TNF-α）水平、细胞间黏附分子1（ICAM-1）和环氧化酶-2（COX-2）。与给予酒精相比，给予Se-AbM显著（p<0.05）降低了血清ALT、AST和MDA水平、肝脏IL-1β和TNF-α水平，以及PMN浸润和ICAM-1、COX-2、iNOS和NF-κB的表达。总之，我们观察到补充Se-AbM可以抑制急性酒精暴露引起的肝脏损伤。
