The William Harvey Research Institute, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Charterhouse Square, London, EC1M 6BQ, UK.
Environ Toxicol Pharmacol. 1999 Mar;7(1):27-31. doi: 10.1016/s1382-6689(98)00046-5.
We have developed a photometric microassay for the assessment of total antioxidant status in plasma at physiological pH and temperature and applied it to evaluate experimental oxidant stress in vivo associated with endothelial dysfunction in vitro. Rat plasma or l-ascorbic acid inhibited the peroxidase-mediated accumulation after 6 min at pH 7.4 and 37°C of ABTS(+) (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical), measured at 405 nm, in a concentration-dependent manner. Plasma total antioxidant status, expressed as the ascorbate equivalent antioxidant concentration, was subsequently found to be significantly reduced in rats treated daily for 7 days in vivo with the oxidant compounds hydroquinone (50 mg/kg i.p.) and triethylenetetramine (100 mg/kg i.p.), either alone or in combination with the glutathione-depleting agent l-buthionine sulfoximine (50 mg/kg i.p). Furthermore, basal endothelial function in isolated aorta was impaired after hydroquinone or triethylenetetramine in a manner aggravated by l-buthionine sulfoximine.
我们开发了一种在生理 pH 值和温度下评估血浆总抗氧化状态的比色微测定法，并将其应用于评估与体外内皮功能障碍相关的体内实验性氧化应激。在 pH 值为 7.4 和 37°C 下，ABTS(+)（2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)自由基）在 6 分钟后被过氧化物酶介导的积累被大鼠血浆或 l-抗坏血酸抑制，其方式呈浓度依赖性。随后发现，在体内每天用氧化化合物对苯二酚（50 mg/kg i.p.）和三乙烯四胺（100 mg/kg i.p.）单独或与谷胱甘肽耗竭剂 l-丁硫氨酸亚砜（50 mg/kg i.p.）联合处理 7 天的大鼠中，血浆总抗氧化状态（以抗坏血酸当量抗氧化浓度表示）显著降低。此外，在苯二酚或三乙烯四胺的作用下，离体主动脉的基础内皮功能受到损害，而 l-丁硫氨酸亚砜则加剧了这种损害。
