Ye Xiaohua, Huai Jiaping, Chen Renpin, Ding Jin, Chen Yanping, Cai Zhenzhai
Department of Gastroenterology and Hepatology, Jinhua Municipal Central Hospital, Jinhua Hospital of Zhejiang University, Jinhua, Zhejiang 321000, P.R. China.
Department of Critical Care Medicine, Jinhua Municipal Central Hospital, Jinhua Hospital of Zhejiang University, Jinhua, Zhejiang 321000, P.R. China.
Exp Ther Med. 2014 Jan;7(1):85-89. doi: 10.3892/etm.2013.1354. Epub 2013 Oct 22.
It has recently been demonstrated that fibrinogen-like protein 2 (fgl2) is expressed on the surface of macrophages, T cells and endothelial cells and directly cleaves prothrombin to thrombin. The present study was designed to examine fgl2 expression in patients with severe acute pancreatitis (SAP) and its correlation with disease progression. Peripheral blood mononuclear cells (PBMCs) were isolated from 25 patients with SAP, 37 patients with mild acute pancreatitis (MAP) and 20 healthy volunteers as controls. Paraffin sections of pancreas were obtained from 18 postoperative patients with SAP between 2003 and 2012. Human fgl2 (hfgl2) gene expression was determined in the PBMCs by real-time PCR. A monoclonal antibody against hfgl2 was applied to detect hfgl2 protein expression in the pancreatic tissues as well as in the PBMCs by immunohistochemical staining. The levels of hfgl2 expression in the PBMCs from the 25 patients with SAP were markedly upregulated compared with the other groups, whereas no significant difference between the MAP group and healthy controls was observed. hfgl2 expression in the PBMCs and pancreatic tissues was detectable through using immunohistochemistry and was demonstrated to be specifically localized to the endothelium of microvessels and inflammatory infiltrative cells in the areas of acute focal, confluent necrosis. There were positive correlations between hfgl2 expression in the PBMCs and the severity of SAP, as indicated by scores of Ranson and Acute Physiology and Chronic Health Evaluation II. The results suggest that hfgl2 is involved in the pathogenesis of SAP and hfgl2 levels may serve as a biomarker during disease progression.
最近有研究表明，纤维蛋白原样蛋白2（fgl2）在巨噬细胞、T细胞和内皮细胞表面表达，并直接将凝血酶原裂解为凝血酶。本研究旨在检测重症急性胰腺炎（SAP）患者中fgl2的表达及其与疾病进展的相关性。从25例SAP患者、37例轻症急性胰腺炎（MAP）患者及20名健康志愿者（作为对照）中分离外周血单个核细胞（PBMC）。获取2003年至2012年间18例术后SAP患者的胰腺石蜡切片。采用实时PCR法测定PBMC中人类fgl2（hfgl2）基因表达。应用抗hfgl2单克隆抗体，通过免疫组化染色检测胰腺组织及PBMC中hfgl2蛋白表达。与其他组相比，25例SAP患者PBMC中hfgl2表达水平显著上调，而MAP组与健康对照组之间未观察到显著差异。通过免疫组化可检测到PBMC和胰腺组织中的hfgl2表达，且证实其特异性定位于急性局灶性、融合性坏死区域的微血管内皮及炎性浸润细胞。PBMC中hfgl2表达与SAP严重程度之间存在正相关，如Ranson评分及急性生理与慢性健康状况评分II所示。结果表明，hfgl2参与了SAP的发病机制，且hfgl2水平可能作为疾病进展过程中的生物标志物。
