Eriksson Cecilia, Masaki Noritaka, Yao Ikuko, Hayasaka Takahiro, Setou Mitsutoshi
Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine ; Medical Mass Spectrometry, Department of Pharmaceutical Biosciences, Uppsala University.
Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine.
Mass Spectrom (Tokyo). 2013;2(Spec Iss):S0022. doi: 10.5702/massspectrometry.S0022. Epub 2013 Apr 15.
As the only imaging method available, Imaging Mass Spectrometry (IMS) can determine both the identity and the distribution of hundreds of molecules on tissue sections, all in one single run. IMS is becoming an established research technology, and due to recent technical and methodological improvements the interest in this technology is increasing steadily and within a wide range of scientific fields. Of the different IMS methods available, matrix-assisted laser desorption/ionization (MALDI) IMS is the most commonly employed. The course at IMSC 2012 in Kyoto covered the fundamental principles and techniques of MALDI-IMS, assuming no previous experience in IMS. This mini review summarizes the content of the one-day course and describes some of the most recent work performed within this research field.
作为唯一可用的成像方法，成像质谱（IMS）能够在一次分析中同时测定组织切片上数百种分子的身份和分布。IMS正在成为一种成熟的研究技术，并且由于近期技术和方法上的改进，该技术在广泛的科学领域内的关注度正稳步上升。在现有的不同IMS方法中，基质辅助激光解吸/电离（MALDI）IMS是最常用的。2012年在京都举办的IMS课程涵盖了MALDI-IMS的基本原理和技术，假定学员此前没有IMS方面的经验。本综述总结了这一天课程的内容，并介绍了该研究领域内一些最新的研究工作。
