Department of Cell Biology and Anatomy, School of Medicine, Hamamatsu University, Shizuoka, Japan.
Biol Pharm Bull. 2012;35(9):1417-24. doi: 10.1248/bpb.b212007.
We have developed a mass microscope in which a microscope is combined with high-resolution matrix assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS). This technique is a powerful tool for investigating the spatial distribution of biomolecules without the need for any time-consuming extraction, purification, and labeling procedures for biological tissue sections. The mass microscope provides clear images with regards to the distribution of hundreds of biomolecules in a single measurement, and also helps in determining the cellular profile of the biological system. In this review, we focus on some of the recent developments in clinical applications and describe how the mass microscope can be employed to assess pathomorphology and pharmacokinetics.
我们开发了一种质谱显微镜，它将显微镜与高分辨率矩阵辅助激光解吸/电离成像质谱（MALDI-IMS）结合在一起。这项技术是一种强大的工具，用于研究生物组织切片中生物分子的空间分布，而无需进行任何耗时的提取、纯化和标记程序。质谱显微镜在单次测量中就能提供关于数百种生物分子分布的清晰图像，并有助于确定生物系统的细胞特征。在这篇综述中，我们重点介绍了一些临床应用的最新进展，并描述了如何使用质谱显微镜来评估病理形态和药代动力学。
