Faecal Escherichia coli mediating transferable multi-antibiotic resistance and undesirable extra-chromosomal genes.

A conjugative R-plasmid PE004, Inc F11, conferring resistance to ampicillin, tetracycline, streptomycin, kanamycin and trimethoprim was obtained from an E. coli serotype 026 isolate from the stool of a child with acute diarrhoea. The R-plasmid PE004 also co-transfers an enteropathogenicity antigen without the production of enterotoxins or manifestation of invasiveness. It is not yet known whether this transferable antigen mediates enterocyte damage with consequent diarrhoea. The R-plasmid was of molecular weight 2.4 megadaltons (3.7 kilobase) with a transfer frequency of 6 x 10(-4) cfu/ml E. coli J53-1. The uncontrolled mediation with antibiotics in cases of acute diarrhoea could select gut bacteria not only possessing R-plasmids conferring resistance to several antibiotics but with associated undesirable extrachromosomal genes.