Shin Dong Ah, Pennant William A, Yoon Do Heum, Ha Yoon, Kim Keung Nyun
Spine and Spinal Cord Institute, Department of Neurosurgery, Yonsei University College of Medicine, Seoul, South Korea.
Acta Neurochir (Wien). 2014 Feb;156(2):297-303. doi: 10.1007/s00701-013-1963-y. Epub 2013 Dec 19.
Spinal cord injury (SCI) is a devastating and irreversible event, and much research using fibroblast growth factor-2 (FGF-2) has been performed to test its capacity to blunt the effects of SCI as well as to provide an environment conducive for SCI repair.
We tested how the in vitro release of FGF-2 from heparin-conjugated poly(L-lactide-co-glycolide) (PLGA)-conjugated nanospheres (HCPNs) affected the growth of human bone marrow-derived mesenchymal stem cells (hBMSCs), as well as the effects of their co-transplantation in an animal model of SCI.
Our results showed that sustained, long-term release of FGF-2 from HCPNs significantly increased hBMSCs proliferation in vitro, and that their co-transplantation following rat SCI lead to increased functional improvement, a greater amount of hBMSCs surviving transplantation, and a greater density of neurofilament-positive cells in the injury epicenter.
These results suggest a proliferative, protective, and neural inductive potential of FGF-2 for transplanted hBMSCs, as well as a possible role for sustained FGF-2 delivery along with hBMSCs transplantation in the injured spinal cord. Future studies will be required to ascertain the safety FGF-2-containing HCPNs before clinical application.
脊髓损伤（SCI）是一种具有毁灭性且不可逆转的事件，人们已开展了许多使用成纤维细胞生长因子-2（FGF-2）的研究，以测试其减轻脊髓损伤影响的能力，并提供有利于脊髓损伤修复的环境。
我们测试了FGF-2从肝素共轭聚（L-丙交酯-共-乙交酯）（PLGA）共轭纳米球（HCPNs）的体外释放如何影响人骨髓间充质干细胞（hBMSCs）的生长，以及它们在脊髓损伤动物模型中的共移植效果。
我们的结果表明，FGF-2从HCPNs的持续、长期释放显著增加了hBMSCs在体外的增殖，并且它们在大鼠脊髓损伤后共移植导致功能改善增加、更多的hBMSCs在移植后存活，以及损伤中心神经丝阳性细胞密度更高。
这些结果表明FGF-2对移植的hBMSCs具有增殖、保护和神经诱导潜力，以及在损伤脊髓中与hBMSCs移植一起持续递送FGF-2的可能作用。在临床应用之前，需要进一步的研究来确定含FGF-2的HCPNs的安全性。
