Medici Maria Cristina, Tummolo Fabio, Guerra Paola, Arcangeletti Maria Cristina, Chezzi Carlo, De Conto Flora, Calderaro Adriana
Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Viale Antonio Gramsci, 14, 43126, Parma, Italy,
Virus Genes. 2014 Apr;48(2):361-5. doi: 10.1007/s11262-013-1026-8. Epub 2013 Dec 19.
Intragenotypic heterogeneity of co-circulating rotaviruses is remarkable. Sequence and phylogenetic analyses of the rotavirus VP7 and VP4 genes were performed on selected human G4P[8] strains identified in Parma, Northern Italy, during 2004-2005 and 2008-2012. All the strains clustered into lineages Ic (VP7) and P[8]-III (VP4) in different subclusters with a nucleotide sequence variation up to 4 %. VP7 and VP4 amino acid sequences of the Italian rotaviruses showed multiple changes with the corresponding reference strains as well as with vaccine viruses in the neutralizing epitopes. There is concern that the progressive intra-lineage diversification in the VP7 and VP4 through the accumulation of point mutations and amino acid differences between vaccine strains and currently circulating rotaviruses could generate, over the years, vaccine-resistant variants.
共同流行的轮状病毒的基因型内异质性十分显著。对2004 - 2005年以及2008 - 2012年期间在意大利北部帕尔马确定的选定人类G4P[8]毒株进行了轮状病毒VP7和VP4基因的序列及系统发育分析。所有毒株在不同亚群中聚为Ic（VP7）和P[8]-III（VP4）谱系，核苷酸序列变异高达4%。意大利轮状病毒的VP7和VP4氨基酸序列与相应参考毒株以及疫苗病毒相比，在中和表位处显示出多处变化。人们担心，随着时间的推移，通过点突变的积累以及疫苗毒株与当前流行的轮状病毒之间的氨基酸差异，VP7和VP4基因在谱系内的逐渐多样化可能会产生疫苗抗性变体。
