Xiao Han, Chatterjee Abhishek, Choi Sei-hyun, Bajjuri Krishna M, Sinha Subhash C, Schultz Peter G
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA) http://schultz.scripps.edu/
Angew Chem Int Ed Engl. 2013 Dec 23;52(52):14080-3. doi: 10.1002/anie.201308137. Epub 2013 Nov 8.
enables the expression of proteins in mammalian cells incorporating one unnatural amino acid (UAA) into multiple sites, as well as two different UAAs into distinct sites in a protein of interest. The utility of this technology was demonstrated by generating a full-length antibody, site-specifically conjugated to a drug and a fluorophore, and characterizing its activity in vitro (see picture). Picture was cropped to fit the available space.
能够在哺乳动物细胞中表达蛋白质，将一种非天然氨基酸（UAA）整合到多个位点，以及将两种不同的UAA整合到目标蛋白质的不同位点。通过生成一种与药物和荧光团位点特异性偶联的全长抗体，并在体外表征其活性，证明了该技术的实用性（见图）。图已裁剪以适应可用空间。
