谷胱甘肽水平、葡萄糖代谢损伤与糖尿病并发症的关系。
The relationship between the level of glutathione, impairment of glucose metabolism and complications of diabetes mellitus.
机构信息
Ismail Hakki Kalkan, MD, Department of Gastroentorology, Kirikkale State Hospital, Kirikkale, Turkey.
Murat Suher, MD, Associate Professor, Department of Internal Medicine, Ataturk Training and Research Hospital, Ankara, Turkey.
出版信息
Pak J Med Sci. 2013 Jul;29(4):938-42. doi: 10.12669/pjms.294.2859.
OBJECTIVE
To investigate whether there is a difference between the subjects with new-onset type 2 diabetes mellitus (DM), impaired glucose tolerance (IGT) and normal fasting blood glucose levels with respect to the level of glutathione (GSH) and the relationship between the presence of complication of diabetes and the level of GSH.
METHODS
Oral Glucose Tolerance Test (OGTT) was performed in IFG patients, with no episode of drug use, who were admitted to hospital. According to the results of the application 30 subjects with type 2 DM, 30 subjects with IGT and 28 subjects with normal blood glucose level were included in the study. Anthropometric measurements and blood pressure values of all subjects were recorded. The biochemical parameters of subjects were studied in the biochemistry laboratory by utilizing Olympus AV-2700. The subjects with diabetic retinopathy and nephropathy were established subsequent to the examination of the retina and 24-hour urine collection test performed to subjects with diagnosis of DM. Levels of GSH in all subjects were measured by enzymatic recycling method.
RESULTS
The mean levels of GSH in subjects with DM were significantly reduced compared with IGT or normal subjects (respectively p=0.02 and p<0.001). Besides, lower levels of GSH were acquired in subjects with IGT compared to normal subjects (p<0.001). The mean levels of GSH in subjects with diabetic retinopathy were lower than the subjects with no established diagnosis of diabetic retinopathy (p<0.001). Similarly, lower levels of GSH (p<0.001) were obtained in microalbuminuric subjects than normoalbuminuric subjects.
CONCLUSIONS
At the end of the study, we came to the conclusion that GSH deficiency was of great significance in the pathogenesis of Diabetes Mellitus.
目的
探讨新诊断 2 型糖尿病（DM）、糖调节受损（IGT）和空腹血糖正常者的谷胱甘肽（GSH）水平是否存在差异，以及糖尿病并发症的发生与 GSH 水平的关系。
方法
对我院住院的 IFG 患者进行口服葡萄糖耐量试验（OGTT），根据 OGTT 结果，纳入新诊断的 2 型 DM 患者 30 例、IGT 患者 30 例和血糖正常者 28 例。记录所有受试者的体格测量和血压值。所有受试者的生化参数均在生化实验室采用 Olympus AV-2700 进行检测。对确诊为 DM 的患者进行眼底检查和 24 小时尿标本采集，以确定是否存在糖尿病视网膜病变和肾病。采用酶循环法测定所有受试者的 GSH 水平。
结果
与 IGT 或正常受试者相比，DM 受试者的 GSH 水平明显降低（分别为 p=0.02 和 p<0.001）。此外，IGT 受试者的 GSH 水平也低于正常受试者（p<0.001）。有糖尿病视网膜病变的受试者的 GSH 水平低于无糖尿病视网膜病变诊断的受试者（p<0.001）。同样，微量白蛋白尿受试者的 GSH 水平也低于正常白蛋白尿受试者（p<0.001）。
结论
研究结束时，我们得出结论，GSH 缺乏在糖尿病发病机制中具有重要意义。
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