表皮生长因子和胰岛素刺激离体大鼠肝细胞核中核孔介导的大分子运输。
Epidermal growth factor and insulin stimulate nuclear pore-mediated macromolecular transport in isolated rat liver nuclei.
作者信息
Schindler M, Jiang L W
出版信息
J Cell Biol. 1987 Apr;104(4):849-53. doi: 10.1083/jcb.104.4.849.
Abstract
Fluorescence photobleaching was used to measure the effect of epidermal growth factor (EGF), insulin, and glucagon on the nuclear transport of fluorescent-labeled dextrans across the nuclear pore complex. EGF and insulin were found to stimulate transport approximately 200%, while boiling these polypeptide growth factors greatly diminished this enhancement activity. Glucagon demonstrated no enhancement effect. The nuclear transport enhancement effects were observed at EGF and insulin concentrations that elicit the various physiological responses, e.g., nanomolar range.
摘要
利用荧光漂白技术来测量表皮生长因子（EGF）、胰岛素和胰高血糖素对荧光标记葡聚糖通过核孔复合体的核转运的影响。发现EGF和胰岛素可使转运刺激约200%，而将这些多肽生长因子煮沸则大大降低了这种增强活性。胰高血糖素未表现出增强作用。在引发各种生理反应的EGF和胰岛素浓度下，如纳摩尔范围，观察到了核转运增强效应。
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