3T3成纤维细胞中的核运输：生长因子、转化和细胞形状的影响。
Nuclear transport in 3T3 fibroblasts: effects of growth factors, transformation, and cell shape.
作者信息
Jiang L W, Schindler M
机构信息
Department of Biochemistry, Michigan State University, East Lansing 48824.
出版信息
J Cell Biol. 1988 Jan;106(1):13-9. doi: 10.1083/jcb.106.1.13.
Abstract
Nucleocytoplasmic transport of fluorescent-labeled macromolecules was investigated in transformed and nontransformed 3T3 fibroblasts. Insulin and epidermal growth factor enhanced transport three-fold after 1-2-h incubation with nontransformed adhering fibroblasts; no enhancement of transport was observed for spherical unattached fibroblasts. The concentration of growth factor for maximal enhancement was 3-10 nM. Nuclear transport for Kirsten murine sarcoma virus-transformed BALB/c 3T3 fibroblasts, however, was maximally enhanced before addition of growth factors; addition of insulin or epidermal growth factor causes no additional transport enhancement. Transformation also minimizes cell shape effects on macromolecular nuclear transport. These results provide evidence that protein growth factors and oncogenic transformation may use a similar mechanism for activation of nuclear transport.
摘要
在转化的和未转化的3T3成纤维细胞中研究了荧光标记大分子的核质运输。胰岛素和表皮生长因子与未转化的贴壁成纤维细胞孵育1 - 2小时后，运输增强了三倍；对于球形的未附着成纤维细胞，未观察到运输增强。最大增强时生长因子的浓度为3 - 10 nM。然而，对于 Kirsten 小鼠肉瘤病毒转化的 BALB/c 3T3 成纤维细胞，在添加生长因子之前核运输就已最大程度增强；添加胰岛素或表皮生长因子不会导致额外的运输增强。转化还使细胞形状对大分子核运输的影响最小化。这些结果提供了证据，表明蛋白质生长因子和致癌转化可能使用类似的机制来激活核运输。
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