分泌抗马传染性贫血病毒单克隆抗体的杂交瘤细胞系。

Hybridoma cell lines secreting monoclonal antibodies against equine infectious anemia virus.

作者信息

Horenstein A L, Glait H M, Koss A

出版信息

J Virol Methods. 1987 Feb;15(3):177-85. doi: 10.1016/0166-0934(87)90096-6.

Abstract

A monoclonal anti-equine infectious anemia virus (anti-EIAV) antibody (1B15) has been generated by fusion of X63 Ag 8.653 myeloma cells and spleen cells from mice hypersensitized with viral antigen p29. Ouchterlony double-diffusion analysis indicated that antibody 1B15 is of the IgG class. The specificity of the immune reaction for p29 was confirmed by cross-over immunoelectrophoresis and disc-gel electrophoresis. MAb 1B15 was used to devise a solid-phase 'capture' RIA for EIAV-p29 antigen. The antigen, bound by 1B15 adsorbed onto wells of flexible microtitre plates, was detected using a rabbit anti-p29 serum followed by a 125I-labelled tracer. The assay was applied to detected using a of virus in horse serum and infected cell culture fluids.

摘要

通过将X63 Ag 8.653骨髓瘤细胞与用病毒抗原p29致敏的小鼠脾细胞融合，产生了一种单克隆抗马传染性贫血病毒（抗EIAV）抗体（1B15）。免疫双扩散分析表明抗体1B15属于IgG类。交叉免疫电泳和圆盘凝胶电泳证实了针对p29的免疫反应的特异性。单克隆抗体1B15用于设计一种针对EIAV-p29抗原的固相“捕获”放射免疫分析。使用兔抗p29血清，随后用125I标记的示踪剂检测吸附在柔性微量滴定板孔上的1B15所结合的抗原。该分析方法用于检测马血清和感染细胞培养液中的病毒。