马单克隆抗体识别马传染性贫血病毒变体上的共同表位。
Equine monoclonal antibodies recognize common epitopes on variants of equine infectious anaemia virus.
作者信息
Perryman L E, O'Rourke K I, Mason P H, McGuire T C
机构信息
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
出版信息
Immunology. 1990 Dec;71(4):592-4.
Equine-murine xenohybridoma cells were produced using SP2/0 murine myeloma cells and splenic lymph node cells obtained from horses infected with 10(6) TCID50 of single cloned variants of equine infectious anaemia virus (EIAV). The xenohybridomas secreted equine IgG monoclonal antibodies reactive with EIAV in enzyme immunoassays employing purified virus. Seven antibodies were studied in detail. They bound to viral glycoproteins (gp90 or gp45) in radioimmunoprecipitation assays, and reacted with homologous EIAV as well as five other cloned variants of EIAV. When evaluated against a single cloned variant of EIAV (EIAV-WSU5), two antibodies bound to different epitopes on gp90. The five remaining antibodies reacted with the same or overlapping epitopes on gp45. None of the antibodies exhibited viral neutralizing activity.
使用SP2/0鼠骨髓瘤细胞和从感染了10(6)半数组织培养感染剂量（TCID50）马传染性贫血病毒（EIAV）单克隆变体的马获取的脾淋巴结细胞，制备了马-鼠异种杂交瘤细胞。这些异种杂交瘤在使用纯化病毒的酶免疫测定中分泌与EIAV反应的马IgG单克隆抗体。对七种抗体进行了详细研究。在放射免疫沉淀试验中，它们与病毒糖蛋白（gp90或gp45）结合，并与同源EIAV以及EIAV的其他五个克隆变体反应。当针对EIAV的一个单克隆变体（EIAV-WSU5）进行评估时，两种抗体与gp90上不同的表位结合。其余五种抗体与gp45上相同或重叠的表位反应。没有一种抗体表现出病毒中和活性。
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