Latimer K S, Duncan J R, Kircher I M
J Comp Pathol. 1987 Jan;97(1):61-72. doi: 10.1016/0021-9975(87)90128-9.
Nuclear segmentation, ultrastructural features and cytochemical staining reactions of blood cells from 7 dogs with the heterozygous form of Pelger-Huët (P-H) anomaly were investigated. Significant nuclear hyposegmentation of granulocytes, monocytes and megakaryocytes was found, indicating a common stem cell defect in the nuclear segmentation or lobulation process. Neutrophils and eosinophils from female P-H dogs lacked sex chromatin drumsticks. Other than the suggestion of nuclear hyposegmentation and the presence of coarse heterochromatin, no ultrastructural abnormalities were discerned. Cytochemical staining by a-naphthyl acetate esterase, alkaline phosphatase, naphthol ASD chloroacetate esterase, periodic acid-Schiff, peroxidase and Sudan black B techniques gave similar results for P-H and control dog leukocytes and platelets.
对7只患有杂合型Pelger-Huët（P-H）异常的犬的血细胞进行了细胞核分割、超微结构特征和细胞化学染色反应研究。发现粒细胞、单核细胞和巨核细胞存在明显的核分叶减少，表明在核分割或分叶过程中存在共同的干细胞缺陷。雌性P-H犬的中性粒细胞和嗜酸性粒细胞缺乏性染色质鼓槌。除了核分叶减少的迹象和粗大异染色质的存在外，未发现超微结构异常。用α-萘乙酸酯酶、碱性磷酸酶、萘酚ASD氯乙酸酯酶、过碘酸-希夫、过氧化物酶和苏丹黑B技术进行细胞化学染色，P-H犬和对照犬的白细胞及血小板得到了相似的结果。
