Ding Aiping, Zhao Wenwen, Shi Xiaoli, Yao Ruyong, Zhou Fang, Yue Lu, Liu Shihai, Qiu Wensheng
Department of Oncology, Affiliated Hospital of Qingdao University School of Medicine, Qingdao, People's Republic of China.
Department of Oncology, Affiliated Hospital of Qingdao University School of Medicine, Qingdao, People's Republic of China ; Qingdao Cancer Hospital, Shibei City, Qingdao, Shandong Province, China.
PLoS One. 2013 Dec 16;8(12):e82136. doi: 10.1371/journal.pone.0082136. eCollection 2013.
NPM, TFF3 and TACC1 are molecular markers that play important roles in cell differentiation. Herein, we investigated their prognostic impact in patients with primary gastric cancer (GC) and determined whether they could be used as markers of more aggressive gastric carcinomas by detecting the extent of expression in human gastric carcinoma samples.
METHODOLOGY/PRINCIPAL FINDINGS: Tumor tissue specimens from 142 GC patients were retrospectively retrieved and immunohistochemically evaluated. Correlations between NPM, TFF3 and TACC1 over-expression and clinicopathologic parameters, and their prognostic values were investigated with χ(2), Kaplan-Meier method, and Cox uni- and multivariate survival models. NPM, TFF3 and TACC1 expression was significantly higher in GC patients with poorly differentiated histologic type than that in patients with well differentiated histologic type. NPM expression was significantly higher in patients with hepatic metastasis or recurrence than that in patients without metastasis. TFF3 expression was significantly higher in patients with positive lymph node metastasis than that in patients with negative lymph node metastasis. Age, lymph node metastasis, and TFF3 and TACC1 over-expression were significantly correlated with low survival (P<0.05, P<0.05, P = 0.005 and P = 0.009, respectively). Multivariate analysis showed that lymph node metastasis and TFF3 and TACC1 over-expression were independent prognostic factors.
TFF3 and TACC1 over-expression in epithelial cells of surgically resected GC tissues was an independent predictor of short survival in GC patients. The prognosis was poorer in patients with positive expression of both TFF3 and TACC1 than that in patients with positive expression of TFF3 or TACC1 alone, or with negative expression of TFF3 and TACC1.
核仁磷酸蛋白（NPM）、三叶因子3（TFF3）和转化酸性卷曲蛋白1（TACC1）是在细胞分化中起重要作用的分子标志物。在此，我们研究了它们对原发性胃癌（GC）患者的预后影响，并通过检测人类胃癌样本中的表达程度来确定它们是否可作为侵袭性更强的胃癌的标志物。
方法/主要发现：回顾性收集142例GC患者的肿瘤组织标本，并进行免疫组化评估。采用χ²检验、Kaplan-Meier法以及Cox单因素和多因素生存模型研究NPM、TFF3和TACC1过表达与临床病理参数之间的相关性及其预后价值。组织学类型为低分化的GC患者中NPM、TFF3和TACC1的表达显著高于高分化患者。有肝转移或复发的患者中NPM表达显著高于无转移患者。淋巴结转移阳性患者中TFF3表达显著高于淋巴结转移阴性患者。年龄、淋巴结转移以及TFF3和TACC1过表达与低生存率显著相关（分别为P<0.05、P<0.05、P = 0.005和P = 0.009）。多因素分析显示，淋巴结转移以及TFF3和TACC1过表达是独立的预后因素。
手术切除的GC组织上皮细胞中TFF3和TACC1过表达是GC患者短期生存的独立预测因素。TFF3和TACC1均阳性表达的患者预后比单独TFF3或TACC1阳性表达或TFF3和TACC1均阴性表达的患者更差。
