Maradonna Francesca, Gioacchini Giorgia, Falcinelli Silvia, Bertotto Daniela, Radaelli Giuseppe, Olivotto Ike, Carnevali Oliana
Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Ancona, Italia.
Dipartimento di Biomedicina Comparata e Alimentazione, Università degli Studi di Padova, Legnaro (Padova), Italia.
PLoS One. 2013 Dec 17;8(12):e83155. doi: 10.1371/journal.pone.0083155. eCollection 2013.
A growing number of studies have been showing that dietary probiotics can exert beneficial health effects in both humans and animals. We previously demonstrated that dietary supplementation with Lactobacillus rhamnosus - a component of the human gut microflora - enhances reproduction, larval development, and the biomineralization process in Danio rerio (zebrafish). The aim of this study was to identify the pathways affected by L. rhamnosus during zebrafish larval development. Our morphological and histochemical findings show that L. rhamnosus accelerates bone deposition through stimulation of the expression of key genes involved in ossification, e.g. runt-related transcription factor 2 (runx2), Sp7 transcription factor (sp7), matrix Gla protein (mgp), and bone gamma-carboxyglutamate (gla) protein (bglap) as well as through inhibition of sclerostin (sost), a bone formation inhibitor. Western blot analysis of mitogen-activated protein kinase 1 and 3-(Mapk1 and Mapk3), which are involved in osteoblast and osteocyte differentiation, documented an increase in Mapk1 16 days post fertilization (dpf) and of Mapk3 23 dpf in individuals receiving L. rhamnosus supplementation. Interestingly, a reduction of sost detected in the same individuals suggests that the probiotic may help treat bone disorders.
越来越多的研究表明，膳食益生菌对人类和动物都能产生有益的健康影响。我们之前证明，膳食补充鼠李糖乳杆菌（人类肠道微生物群的一个组成部分）可促进斑马鱼的繁殖、幼体发育和生物矿化过程。本研究的目的是确定鼠李糖乳杆菌在斑马鱼幼体发育过程中影响的途径。我们的形态学和组织化学研究结果表明，鼠李糖乳杆菌通过刺激参与骨化的关键基因（如与 runt 相关的转录因子 2（runx2）、Sp7 转录因子（sp7）、基质 Gla 蛋白（mgp）和骨γ-羧基谷氨酸（gla）蛋白（bglap））的表达，以及通过抑制骨形成抑制剂硬化蛋白（sost）来加速骨沉积。对参与成骨细胞和骨细胞分化的丝裂原活化蛋白激酶 1 和 3（Mapk1 和 Mapk3）的蛋白质印迹分析表明，在接受鼠李糖乳杆菌补充的个体中，受精后 16 天（dpf）Mapk1 增加，23 dpf 时 Mapk3 增加。有趣的是，在同一批个体中检测到的 sost 减少表明，这种益生菌可能有助于治疗骨骼疾病。
