Key Laboratory of Animal Reproductive Physiology and Embryo Biotechnology, Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, People's Republic of China.
Reproduction. 2013 Nov 16;147(1):53-64. doi: 10.1530/REP-13-0141. Print 2014 Jan.
To increase the knowledge of probiotic effects on zebrafish (Danio rerio), we compare the effects of two probiotic strains, Lactobacillus rhamnosus CICC 6141 (a highly adhesive strain) and Lactobacillus casei BL23 (a weakly adhesive strain), on zebrafish reproduction and their offsprings' innate level of immunity to water-borne pathogens. During probiotics treatments from 7 to 28 days, both the Lactobacillus strains, and especially L. casei BL23, significantly increased fecundity in zebrafish: higher rates of egg ovulation, fertilization, and hatching were observed. Increased densities of both small and large vitellogenic follicles, seen in specimens fed either Lactobacillus strain, demonstrated accelerated oocyte maturation. Feeding either strain of Lactobacillus upregulated gene expression of leptin, kiss2, gnrh3, fsh, lh, lhcgr, and paqr8, which were regarded to enhance fecundity and encourage oocyte maturation. Concomitantly, the gene expression of bmp15 and tgfb1 was inhibited, which code for local factors that prevent oocyte maturation. The beneficial effects of the Lactobacillus strains on fecundity diminished after feeding of the probiotics was discontinued, even for the highly adhesive gut Lactobacillus strain. Administering L. rhamnosus CICC 6141 for 28 days was found to affect the innate immunity of offspring derived from their parents, as evinced by a lower level of alkaline phosphatase activity in early larval stages. This study highlights the effects of probiotics both upon the reproductive process and upon the offsprings' immunity during early developmental stages.
为了增加人们对益生菌对斑马鱼（Danio rerio）影响的认识，我们比较了两种益生菌菌株，即鼠李糖乳杆菌 CICC 6141（一种高黏附性菌株）和干酪乳杆菌 BL23（一种低黏附性菌株），对斑马鱼繁殖及其后代对水传播病原体固有免疫水平的影响。在从第 7 天到第 28 天的益生菌处理期间，两种乳酸菌，特别是干酪乳杆菌 BL23，显著增加了斑马鱼的繁殖力：观察到排卵、受精和孵化的比例更高。无论投喂哪种乳酸菌，小型和大型卵黄生成滤泡的密度都增加了，这表明卵母细胞成熟加速。投喂任何一种乳酸菌都会上调瘦素、kiss2、gnrh3、fsh、lh、lhcgr 和 paqr8 的基因表达，这些基因被认为可以提高繁殖力并促进卵母细胞成熟。同时，bmp15 和 tgfb1 的基因表达被抑制，它们编码阻止卵母细胞成熟的局部因子。停止投喂益生菌后，乳酸菌对繁殖力的有益影响减弱，即使是对高黏附性肠道乳酸菌也是如此。连续 28 天投喂鼠李糖乳杆菌 CICC 6141 会影响其亲代后代的先天免疫力，表现在早期幼虫阶段碱性磷酸酶活性较低。这项研究强调了益生菌对生殖过程和早期发育阶段后代免疫力的影响。
