Miccoli Andrea, Gioacchini Giorgia, Maradonna Francesca, Benato Francesca, Skobo Tatjana, Carnevali Oliana
Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy.
Cell Physiol Biochem. 2015;35(5):1706-18. doi: 10.1159/000373983. Epub 2015 Mar 19.
BACKGROUND/AIMS: Probiotic strains have been recognized to exert important roles in many biological systems, including immune response, growth, development and reproduction. However, to date, no studies have focused either on the relation among probiotics and maternal factors or on probiotics' ability to qualitatively and/or quantitatively modulate maternal transcripts.
In this study, the effects of Lactobacillus rhamnosus administered to parental fish on the control of maternal factors involved in autophagic, apoptotic and dorsalizing processes during zebrafish embryo development were assessed through q-PCRs, WMISH and TUNEL assay.
The results we obtained show that probiotic induced significant changes in both maternal and zygotic mRNA levels involved in embryo development. The maternal autophagy-regulating genes herein investigated--ambra1a, ambra1b, beclin, lc3-, as well as those involved in the apoptotic process--caspase3, bcl2, bax--were modulated in disfavor and favor of the treated group, respectively. Also, the key transcripts ruling the dorsalizing process--goosecoid and chordin--were subject to a significant regulation of their gene expression.
The results we acquired demonstrated that parentally administered Lactobacillus rhamnosus is able to modulate important physiological processes involved in zebrafish embryo development.
背景/目的：益生菌菌株已被认为在许多生物系统中发挥重要作用，包括免疫反应、生长、发育和繁殖。然而，迄今为止，尚无研究关注益生菌与母体因素之间的关系，也没有研究关注益生菌定性和/或定量调节母体转录本的能力。
在本研究中，通过定量聚合酶链反应（q-PCR）、全胚胎原位杂交（WMISH）和末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）检测，评估给亲代鱼投喂鼠李糖乳杆菌对斑马鱼胚胎发育过程中自噬、凋亡和背化过程相关母体因素调控的影响。
我们获得的结果表明，益生菌诱导了胚胎发育过程中母体和合子mRNA水平的显著变化。本研究中所检测的母体自噬调节基因——自噬相关基因1a（ambra1a）、自噬相关基因1b（ambra1b）、贝克林蛋白（beclin）、微管相关蛋白1轻链3（lc3-），以及参与凋亡过程的基因——半胱天冬酶3（caspase3）、B细胞淋巴瘤/白血病-2基因（bcl2）、Bax蛋白（bax），分别朝着不利于和有利于处理组的方向被调节。此外，控制背化过程的关键转录本——鹅膏蕈氨酸（goosecoid）和脊索蛋白（chordin）——的基因表达也受到了显著调控。
我们获得的结果表明，给亲代投喂鼠李糖乳杆菌能够调节斑马鱼胚胎发育过程中重要的生理过程。
