人I型T细胞白血病病毒转化的细胞中一种白细胞介素2受体样蛋白的酪氨酸磷酸化
Tyrosine phosphorylation of an interleukin 2 receptor-like protein in cells transformed by human T cell leukemia virus type I.
作者信息
Ogawa R, Sugamura K, Watanabe Y
出版信息
J Exp Med. 1987 Apr 1;165(4):959-69. doi: 10.1084/jem.165.4.959.
Abstract
Three interleukin 2 (IL-2)-independent human T cell lines transformed with human T cell leukemia virus type I were analyzed for o-phosphotyrosine-containing proteins (Ptyr proteins). Membrane and intracellular immunofluorescence was positive with antibody to Ptyr (Ptyr antibody). 10 size classes of Ptyr proteins with molecular masses of 81-28 kD were isolated with Ptyr antibodies. Among these, proteins of 64 kD (pI 4.5 and 4.8-5.3) and 45 kD (pI 4.3) were found on the outer cell surface. The Ptyr protein of 64 kD (pI 4.5) had the characteristics of the IL-2 receptor (IL-2-R) in that it was recognized by two monoclonal antibodies directed against different epitopes on the IL-2-R.
摘要
对三种用人T细胞白血病病毒I型转化的白细胞介素2（IL-2）非依赖性人T细胞系进行了含邻磷酸酪氨酸蛋白（Ptyr蛋白）分析。用抗Ptyr抗体（Ptyr抗体）进行膜和细胞内免疫荧光检测呈阳性。用Ptyr抗体分离出10种分子量为81 - 28 kD的Ptyr蛋白大小类别。其中，64 kD（pI 4.5和4.8 - 5.3）和45 kD（pI 4.3）的蛋白存在于细胞外表面。64 kD（pI 4.5）的Ptyr蛋白具有IL-2受体（IL-2-R）的特征，因为它能被两种针对IL-2-R上不同表位的单克隆抗体识别。
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