Department of Dermatology, Ruhr-University Bochum, Gudrunstraße 56, 44791, Bochum, Germany.
Br J Dermatol. 2014 Jun;170(6):1358-61. doi: 10.1111/bjd.12797.
High-definition optical coherence tomography (HD-OCT) scanners have recently been developed, providing significantly higher resolution than conventional OCT.
To assess the relationship between recently defined histopathological HD-OCT correlates of basal cell carcinomas (BCC) and possible predictors for the most common tumour subtypes.
For HD-OCT imaging, we used the Skintell(®) device. Twenty-five BCCs were histopathologically confirmed (including both vertical and horizontal haematoxylin and eosin and Alcian blue sectioning) and correlated with HD-OCT images.
In the en face mode, lobulated nodules were seen in 21/25 BCCs (84%), peripheral rimming in 18/25 (72%), epidermal disarray in 18/25 (72%) and variably refractile stroma in 22/25 (88%). In the slice imaging mode, we observed destruction of layering in 19/25 (76%) BCCs. In both the slice and en face modes a significant correlation was observed between peritumoral rimming and grey/dark oval structures and lobulated nodules. Alcian blue stains showed peritumoral mucin deposits correlating with peripheral rimming around the tumour nodules. In a logistic regression model, we did not observe significant independent micromorphological HD-OCT predictors for either the solid or superficial BCC subtypes.
In agreement with recent studies we have demonstrated that HD-OCT using the slice and en face imaging modes can visualize histopathological correlates of BCC, and potentially aid noninvasive diagnostics. However, using HD-OCT correlation it was not possible to predict the superficial or solid BCC subtypes. For the first time we have shown that peripheral rimming in HD-OCT correlates with peritumoral mucin deposition.
高清光学相干断层扫描（HD-OCT）扫描仪最近已经开发出来，其提供的分辨率明显高于传统 OCT。
评估最近定义的基底细胞癌（BCC）的组织病理学 HD-OCT 相关物与最常见肿瘤亚型的可能预测因子之间的关系。
对于 HD-OCT 成像，我们使用了 Skintell(®) 设备。25 例 BCC 经组织病理学证实（包括垂直和水平苏木精和伊红以及阿尔辛蓝切片），并与 HD-OCT 图像相关联。
在正面模式下，25 例 BCC 中有 21 例（84%）可见分叶状结节，18 例（72%）可见周边边缘，18 例（72%）可见表皮紊乱，22 例（88%）可见不同程度的折射基质。在切片成像模式下，我们观察到 19 例（76%）BCC 存在分层破坏。在切片和正面两种模式下，肿瘤周围边缘与灰色/暗卵圆形结构和分叶状结节之间均存在显著相关性。阿尔辛蓝染色显示肿瘤周围有粘蛋白沉积，与肿瘤结节周围的周边边缘相对应。在逻辑回归模型中，我们没有观察到对于实性或浅表性 BCC 亚型有显著独立的微观形态学 HD-OCT 预测因子。
与最近的研究一致，我们已经证明，使用切片和正面成像模式的 HD-OCT 可以可视化 BCC 的组织病理学相关物，并有可能辅助非侵入性诊断。然而，使用 HD-OCT 相关性并不能预测浅表性或实性 BCC 亚型。我们首次表明，HD-OCT 中的周边边缘与肿瘤周围粘蛋白沉积相关。
