Huland H, Arndt R, Huland E, Loening T, Steffens M
J Urol. 1987 Apr;137(4):654-9. doi: 10.1016/s0022-5347(17)44166-8.
Monoclonal antibodies directed against tumor-associated antigens of bladder carcinoma were used to identify tumor cells in bladder washout specimens of 40 patients with bladder carcinoma (group 1), 41 with no bladder disease or with urinary tract infections (group 2), 41 who received long-term mitomycin C instillation therapy after excision of the tumors (group 3) and 39 who received no prophylaxis after excision of the tumors (group 4). In all groups the same bladder washout specimen was used for standard urinary cytological and immunocytological tests. True positive results were obtained in 90 per cent of the patients in group 1 according to our immunocytological criteria and in 43 per cent according to standard cytology studies. No urine specimens in group 2 (controls) were immunocytologically positive, while 16 of 41 in group 3 and 17 of 39 in group 4 were positive immunocytologically but only 4 and 5, respectively, were positive according to standard cytology studies. Further followup of these patients will show whether cells positive for monoclonal antibody 486 P 3/12 will permit early detection of recurrent bladder cancer and whether one can identify patients who require prophylaxis after removal of the superficial bladder tumors.
针对膀胱癌肿瘤相关抗原的单克隆抗体被用于在40例膀胱癌患者（第1组）、41例无膀胱疾病或患有尿路感染的患者（第2组）、41例肿瘤切除后接受长期丝裂霉素C灌注治疗的患者（第3组）以及39例肿瘤切除后未接受预防治疗的患者（第4组）的膀胱冲洗标本中识别肿瘤细胞。在所有组中，相同的膀胱冲洗标本用于标准尿液细胞学和免疫细胞学检测。根据我们的免疫细胞学标准，第1组中90%的患者获得了真阳性结果，而根据标准细胞学研究，这一比例为43%。第2组（对照组）的尿液标本在免疫细胞学上均为阴性，而第3组41例中有16例、第4组39例中有17例在免疫细胞学上呈阳性，但根据标准细胞学研究，分别只有4例和5例呈阳性。对这些患者的进一步随访将显示，单克隆抗体486 P 3/12阳性的细胞是否能使复发性膀胱癌得以早期检测，以及是否能识别出浅表性膀胱肿瘤切除后需要预防治疗的患者。
