Arndt R, Dürkopf H, Huland H, Donn F, Loening T, Kalthoff H
J Urol. 1987 Apr;137(4):758-63. doi: 10.1016/s0022-5347(17)44205-4.
We present two monoclonal antibodies (mab), Mano 4/4 and 486 P3/12, directed against tumor-associated antigens of different subpopulations of transitional cell bladder carcinoma tumors. A detailed description is given concerning their reactivity patterns in an ELISA assay against different cell lines. Using immunohistochemical staining against normal bladder mucosa, mab Mano 4/4 reacts with distinct cells in the basal layer and mab 486 P 3/12 recognizes single cells in the superficial layer. Analysing different transitional-cell carcinomas, mab Mano 4/4 reacts with 17 of 20 and mab 486 P 3/12 with 17 of 19 bladder tumors. It is emphasized that both mabs may add new information in respect to a better characterization of the heterogeneity of bladder carcinoma. A biochemical characterization of the mabs and their corresponding antigens is given. Mab Mano 4/4 is directed against a 28 kD glycoprotein and mab 486 P 3/12 is reacting with a 200 kD glycoprotein belonging to the family of CEA-like proteins.
我们展示了两种单克隆抗体（mab），Mano 4/4和486 P3/12，它们针对移行细胞膀胱癌不同亚群的肿瘤相关抗原。详细描述了它们在针对不同细胞系的ELISA检测中的反应模式。使用针对正常膀胱黏膜的免疫组织化学染色，mab Mano 4/4与基底层的不同细胞发生反应，而mab 486 P 3/12识别表层的单个细胞。分析不同的移行细胞癌，mab Mano 4/4与20例中的17例膀胱肿瘤发生反应，mab 486 P 3/12与19例中的17例膀胱肿瘤发生反应。需要强调的是，这两种单克隆抗体在更好地表征膀胱癌的异质性方面可能会提供新的信息。还给出了单克隆抗体及其相应抗原的生化特征。mab Mano 4/4针对一种28 kD糖蛋白，mab 486 P 3/12与一种属于CEA样蛋白家族的200 kD糖蛋白发生反应。
