Fortunato Adriana, Boni Raffaele, Leo Rita, Nacchia Giuseppina, Liguori Francesca, Casale Sofia, Bonassisa Paolo, Tosti Elisabetta
IDF Center, Piazza Municipio, 4, Naples, Italy.
Department of Sciences, University of Basilicata, Potenza, Italy.
Reprod Biomed Online. 2016 Feb;32(2):154-61. doi: 10.1016/j.rbmo.2015.10.009. Epub 2015 Nov 10.
In this retrospective study of 873 men enrolled for assisted reproduction techniques, relationships between sperm quality parameters, motile sperm organelle morphology examination (MSOME), DNA damage and live birth rate were evaluated. The presence of vacuoles in the sperm heads was detected by MSOME. Either chromatin decondensation or DNA fragmentation was used to study DNA damage. Results show that age significantly affected some of the examined parameters. In particular, sperm concentration was positively correlated (R = 0.088; P = 0.01) and chromatin decondensation was negatively correlated (R = -0.102; P = 0.003) with age. Furthermore, live birth rate was significantly lower in men aged 40 years or older (P < 0.02) compared with the younger age groups. The presence of sperm head vacuoles was not associated with head morphology, main sperm quality parameters, DNA fragmentation and live birth rate. Considering sperm heads in relation to the shape (normal/abnormal) and vacuoles (presence/absence), no significant variations in the occurrence of vacuoles in either normal or abnormal heads were found. These data suggest that vacuoles are physiological features that do not alter sperm functionality, and it seems that MSOME is not necessary for increasing the success of assisted reproduction techniques.
在这项对873名接受辅助生殖技术的男性进行的回顾性研究中，评估了精子质量参数、活动精子细胞器形态检查（MSOME）、DNA损伤与活产率之间的关系。通过MSOME检测精子头部空泡的存在情况。采用染色质解聚或DNA片段化来研究DNA损伤。结果显示，年龄对一些检测参数有显著影响。具体而言，精子浓度与年龄呈正相关（R = 0.088；P = 0.01），染色质解聚与年龄呈负相关（R = -0.102；P = 0.003）。此外，40岁及以上男性的活产率显著低于年轻年龄组（P < 0.02）。精子头部空泡的存在与头部形态、主要精子质量参数、DNA片段化及活产率均无关联。考虑精子头部的形状（正常/异常）和空泡（存在/不存在），正常或异常头部中空泡出现的情况均未发现显著差异。这些数据表明，空泡是不改变精子功能的生理特征，而且似乎MSOME对于提高辅助生殖技术的成功率并非必要。
