Raivich G, Kreutzberg G W
Neuroscience. 1987 Jan;20(1):23-36. doi: 10.1016/0306-4522(87)90003-0.
Although beta-nerve growth factor is primarily known for its trophic role in the peripheral nervous system, recent reports have also revealed an inductive effect of beta-nerve growth factor on the cholinergic metabolism of the forebrain. To learn more about the significance and location of beta-nerve growth factor action in the central nervous system, the distribution of [125I]beta-nerve growth factor binding sites was studied by using the method of in situ receptor autoradiography and compared with the distribution of acetylcholinesterase, a sensitive enzyme marker of cholinergic neurons. The autoradiographic studies demonstrated strong, specific and saturable [125I]beta-nerve growth factor binding to several neuronal groupings in the forebrain and brainstem. beta-Nerve growth factor binding sites and strong acetylcholinesterase reactivity were jointly distributed in the forebrain on the medial septal nucleus, the diagonal band of Broca, the magnocellular basal nucleus and in the striatum. In the brainstem, beta-nerve growth factor binding sites were located on a number of neuronal groups in the reticular formation, the dorsolateral lemniscus and the cochlear nuclei. In contrast to the forebrain, less correlation was found with the distribution of acetylcholinesterase; no beta-nerve growth factor receptor expression was recorded on the cholinergic motor nuclei of the brainstem, while specific [125I]beta-nerve growth factor labeling could be located on the non-cholinergic cochlear nuclei. The present autoradiographic studies reveal a variety of tentatively beta-nerve growth factor receptor-positive neurons in the central nervous system. While strong correlation between the cholinergic metabolism and the presence of specific beta-nerve growth factor binding is demonstrated in the forebrain, this observation could not be extended to the brainstem, indicating the chemical diversity of central beta-nerve growth factor receptor-positive neurons.
虽然β-神经生长因子主要因其在外周神经系统中的营养作用而为人所知，但最近的报告也揭示了β-神经生长因子对前脑胆碱能代谢的诱导作用。为了更多地了解β-神经生长因子在中枢神经系统中作用的意义和位置，采用原位受体放射自显影法研究了[125I]β-神经生长因子结合位点的分布，并与乙酰胆碱酯酶（一种胆碱能神经元的敏感酶标志物）的分布进行了比较。放射自显影研究表明，[125I]β-神经生长因子与前脑和脑干中的几个神经元群有强烈、特异性和饱和性的结合。β-神经生长因子结合位点和强烈的乙酰胆碱酯酶反应性共同分布在前脑的内侧隔核、布罗卡斜带、大细胞基底核和纹状体中。在脑干中，β-神经生长因子结合位点位于网状结构、背外侧丘系和蜗神经核的一些神经元群上。与前脑不同，与乙酰胆碱酯酶的分布相关性较小；在脑干的胆碱能运动核上未记录到β-神经生长因子受体表达，而在非胆碱能蜗神经核上可定位到特异性的[125I]β-神经生长因子标记。目前的放射自显影研究揭示了中枢神经系统中各种初步的β-神经生长因子受体阳性神经元。虽然在前脑中证明了胆碱能代谢与特异性β-神经生长因子结合的存在之间有很强的相关性，但这一观察结果不能扩展到脑干，这表明中枢β-神经生长因子受体阳性神经元的化学多样性。
