College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, PR China.
College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, PR China.
Int J Biol Macromol. 2014 Mar;64:313-8. doi: 10.1016/j.ijbiomac.2013.12.019. Epub 2013 Dec 18.
Peptidoglycan recognition proteins (PGRPs) are secreted innate immunity pattern recognition molecules. In this study, a new peptidoglycan recognition protein 1 named cnPGRP1 was isolated from an antler base of sika deer Cervus nippon. The antler base antimicrobial proteins (AAP) were subjected to consecutive chromatographic methods connected to Sephadex G-25 gel filtration column (CM) anion-exchange column, and RP-HPLC. The molecular weight of cnPGRP1 was 17.2 kDa under SDS-PAGE, and peptide mass fingerprint analysis by MALDI-TOF-MS as peptidoglycan recognition protein 1 matched to Dasypus novemcinctus. The matched amino acids sequences were RLYEIIQKWPHYRA. Both Gram-positive and Gram-negative bacteria can be killed by cnPGRP1 in the 50-250 μg/mL range through in vitro. Furthermore, cnPGRP1 has been found to bind Gram-positive bacteria, Gram-negative bacteria, and even fungus.
肽聚糖识别蛋白（PGRPs）是分泌型先天免疫模式识别分子。本研究从梅花鹿（Cervus nippon）鹿茸基部分离到一种新的肽聚糖识别蛋白 1，命名为 cnPGRP1。鹿茸抗菌蛋白（AAP）经连续层析方法连接到 Sephadex G-25 凝胶过滤柱（CM）阴离子交换柱和 RP-HPLC。SDS-PAGE 下 cnPGRP1 的分子量为 17.2 kDa，MALDI-TOF-MS 的肽质量指纹图谱分析表明，Dasypus novemcinctus 的肽聚糖识别蛋白 1 与 cnPGRP1 相匹配。匹配的氨基酸序列为 RLYEIIQKWPHYRA。cnPGRP1 在 50-250 μg/mL 范围内通过体外实验可以杀死革兰氏阳性菌和革兰氏阴性菌。此外，cnPGRP1 已被发现可以结合革兰氏阳性菌、革兰氏阴性菌，甚至真菌。
