College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, China.
Pharm Biol. 2022 Dec;60(1):1077-1087. doi: 10.1080/13880209.2022.2068617.
Deer antler based active ingredients are known to have certain anti-inflammatory and antioxidant activities. However, its potential hepatoprotective effect remains unclear.
This article reports the hepatoprotective effect of protein components in deer antler bases (R1) on lipopolysaccharide/d-galactosamine (LPS/d-GalN)-induced acute liver injury (ALI) in mice, and explores its possible mechanism.
The four separated and purified protein components of deer antler bases were screened and verified by the RAW264.7 cell inflammation model. In the experiment of LPS/d-GalN-induced ALI in mice, ALT, AST, SOD, CAT, GSH and MDA were detected. The liver histopathology was analysed, the COX-2 and iNOS proteins were analysed by immunohistochemistry, and 4-HNE was analysed by immunofluorescence staining. In addition, the effects on the MAPK pathway and NF-κB/IκB-α pathway in liver proteins were explored.
With isolated RA protein fraction pre-treated RAW264.7 cells, NO production decreased by 35.3% compared with the model group. The experimental results of ALI in mice induced by LPS/d-GalN show that R1 protein components can protect mice from ALI through anti-inflammatory and anti-oxidative stress effects and reduce liver pathological damage in mice. The results also indicate that the R1 protein component may protect the liver by inhibiting the activation of the MAPK pathway and the NF-κB/IκB-α pathway induced by LPS/d-GalN.
The separated and purified R1 protein component of deer antler base has a good protective effect on LPS/d-GalN-induced liver injury, and may become a potential material for protecting against liver injury.
鹿茸中含有的某些活性成分具有抗炎和抗氧化作用。然而，其潜在的保肝作用尚不清楚。
本文报道了鹿茸基中蛋白质成分（R1）对脂多糖/半乳糖胺（LPS/d-GalN）诱导的小鼠急性肝损伤（ALI）的保护作用，并探讨了其可能的机制。
采用 RAW264.7 细胞炎症模型筛选和验证鹿茸基中分离和纯化的 4 种蛋白质成分。在 LPS/d-GalN 诱导的小鼠 ALI 实验中，检测 ALT、AST、SOD、CAT、GSH 和 MDA。分析肝组织病理学，用免疫组化法分析 COX-2 和 iNOS 蛋白，用免疫荧光染色法分析 4-HNE。此外，还探讨了其对肝蛋白中 MAPK 通路和 NF-κB/IκB-α通路的影响。
与模型组相比，用分离的 RA 蛋白部分预处理 RAW264.7 细胞后，NO 产生减少了 35.3%。LPS/d-GalN 诱导的小鼠 ALI 实验结果表明，R1 蛋白成分可通过抗炎和抗氧化应激作用保护小鼠免受 ALI，并减轻小鼠肝脏的病理损伤。结果还表明，R1 蛋白成分可能通过抑制 LPS/d-GalN 诱导的 MAPK 通路和 NF-κB/IκB-α 通路的激活来保护肝脏。
鹿茸基中分离和纯化的 R1 蛋白成分对 LPS/d-GalN 诱导的肝损伤具有良好的保护作用，可能成为一种潜在的护肝物质。
