Wieme Anneleen D, Spitaels Freek, Aerts Maarten, De Bruyne Katrien, Van Landschoot Anita, Vandamme Peter
Laboratory of Biochemistry and Brewing, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
Appl Environ Microbiol. 2014 Feb;80(4):1528-38. doi: 10.1128/AEM.03708-13. Epub 2013 Dec 20.
The effect of the growth medium used on the matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectra generated and its consequences for species and strain level differentiation of acetic acid bacteria (AAB) were determined by using a set of 25 strains. The strains were grown on five different culture media that yielded a total of more than 600 mass spectra, including technical and biological replicates. The results demonstrate that the culture medium can have a profound effect on the mass spectra of AAB as observed in the presence and varying signal intensities of peak classes, in particular when culture media do not sustain optimal growth. The observed growth medium effects do not disturb species level differentiation but strongly affect the potential for strain level differentiation. The data prove that a well-constructed and robust MALDI-TOF mass spectrometry identification database should comprise mass spectra of multiple reference strains per species grown on different culture media to facilitate species and strain level differentiation.
使用一组25株菌株，确定了所用生长培养基对生成的基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱的影响及其对醋酸菌（AAB）物种和菌株水平分化的影响。这些菌株在五种不同的培养基上生长，总共产生了600多个质谱，包括技术和生物学重复。结果表明，培养基可对AAB的质谱产生深远影响，如在峰类的存在和信号强度变化中观察到的那样，特别是当培养基不能维持最佳生长时。观察到的生长培养基效应不会干扰物种水平的分化，但会强烈影响菌株水平分化的潜力。数据证明，一个构建良好且强大的MALDI-TOF质谱鉴定数据库应包含每个物种在不同培养基上生长的多个参考菌株的质谱，以促进物种和菌株水平的分化。
